A comparison of cotton and flocked swabs for vaginal self-sample collection

Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval. We recruit...

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Bibliographic Details
Published in:International journal of women's health 2018-01, Vol.10, p.229-236
Main Authors: Viviano, Manuela, Willame, Alexia, Cohen, Marie, Benski, Anne-Caroline, Catarino, Rosa, Wuillemin, Christine, Tran, Phuong Lien, Petignat, Patrick, Vassilakos, Pierre
Format: Article
Language:English
Subjects:
Analysis
Cancer
Cancer research
Cancer screening
Cellular biology
Cervical cancer
cervical cytology
cervical neoplasia
Colposcopy
Cotton
Deoxyribonucleic acid
Developing countries
Diagnosis
DNA
DNA fingerprints
EDTA
Gynecology
Human papillomavirus
LDCs
Medical screening
Original Research
Papillomavirus
Papillomavirus infections
Polymerase chain reaction
screening
Sex crimes
Streptococcus infections
Systematic review
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Summary:Vaginal self-sampling for human papillomavirus (HPV) testing has recently been proposed to optimize cervical cancer screening coverage. The objective of this study was to compare the performance of self-taken samples using flocked and cotton swabs for HPV detection and cellular retrieval. We recruited women aged 21-65 years, referred to colposcopy at the Division of Gynecology of the Geneva University Hospitals between May and September 2016. Each participant collected 2 vaginal samples: 1 with a cotton swab and 1 with a flocked swab. A 1:1 randomization determined the order in which the 2 samples were taken. The swabs were introduced into a 20 mL PreservCyt vial. Real-time polymerase chain reaction analysis using the Anyplex™ II HPV HR assay, cytofluorometric analysis and cytological cell counting were performed on each sample. A total of 119 participants were recruited in the study. Their mean ± standard deviation age was 35.1±8.9 years. The HPV prevalence was 29.7% and 38.1% according to the cotton and flocked swab, respectively ( =0.006). The mean number of cells collected per milliliter according to cytofluorometry was 96,726.6 with the cotton swab and 425,544.3 with the flocked swab (
ISSN:1179-1411
1179-1411
DOI:10.2147/IJWH.S157897