Fusobacterium periodonticum BCT protein targeting glucose metabolism to promote the epithelial-mesenchymal transition of esophageal cancer cells by lactic acid

The cancer microbiota was considered the main risk factor for cancer progression. We had proved that Fusobacterium periodonticum (F.p) was higher abundance in Esophageal cancer(EC)tissues. Bioinformation analysis found that BCT was a key virulence protein of F.p. However, little is known about the r...

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Bibliographic Details
Published in:Journal of translational medicine 2024-04, Vol.22 (1), p.401-401, Article 401
Main Authors: Guo, Xinxin, Wan, Ping, Shen, Weitao, Sun, Mingjun, Peng, Zhenyan, Liao, Yinghao, Huang, Yang, Liu, Ran
Format: Article
Language:English
Subjects:
Analysis
Bacterial Proteins - metabolism
Care and treatment
Cell Line, Tumor
Cell Movement
Cholecystokinin
Composition
Development and progression
Dextrose
Diagnosis
Dosage and administration
EMT
Enzyme-linked immunosorbent assay
Epithelial-Mesenchymal Transition
Esophageal cancer
Esophageal Neoplasms - metabolism
Esophageal Neoplasms - pathology
Fusobacterium - metabolism
Fusobacterium periodonticum
Gene Expression Regulation, Neoplastic
Glucose
Glucose - metabolism
Glycolysi
Gram-negative bacteria
Health aspects
Humans
Lactic acid
Lactic Acid - metabolism
Metabolites
Microbiota (Symbiotic organisms)
Neoplasm Invasiveness
Recombinant proteins
Risk factors
Stem cells
TLR4/Akt/HIF-1α
Tryptophan
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Summary:The cancer microbiota was considered the main risk factor for cancer progression. We had proved that Fusobacterium periodonticum (F.p) was higher abundance in Esophageal cancer(EC)tissues. Bioinformation analysis found that BCT was a key virulence protein of F.p. However, little is known about the role and mechanism of BCT in EC. This study aimed to recognize the key virulence protein of F.p and explore the mechanism of BCT in promoting EC. We constructed a eukaryotic expression vector and purified the recombinant protein BCT. CCK8 used to analyzed the activity of EC after treated by different concentration of BCT. UPLC-MS/MS and ELISA used to detect the metabonomics and metabolites. The ability of migration and invasion was completed by transwell assay. RT-QPCR, WB used to analyze the expression of relevant genes. Our data showed that BCT was higher expression in EC tumor tissues (p 
ISSN:1479-5876
1479-5876
DOI:10.1186/s12967-024-05157-z