Expression of CD40 and CD192 in Classical Monocytes in Multiple Sclerosis Patients Assessed with Transcranial Magnetic Stimulation

• Published in: Biomedicines 2023-10, Vol.11 (10), p.2870
• Main Authors: Režić Mužinić, Nikolina, Markotić, Anita, Pavelin, Sanda, Polančec, Denis, Buljubašić Šoda, Maja, Bralić, Antonia, Šoda, Joško, Mastelić, Angela, Mikac, Una, Jerković, Ana, Rogić Vidaković, Maja
• Format: Article
• Language: English
• Subjects: Antibodies; B cells; Biomarkers; Blood; CD14 antigen; CD14++CD16− monocytes; CD14+CD16++ monocytes; CD16 antigen; CD192; CD40; CD40 antigen; Data collection; Development and progression; Disease; Drugs; Flow cytometry; Immunology; Inflammation; Interferon beta-1b; Latency; Magnetic fields; Medical colleges; Medical research; Medicine, Experimental; Monocytes; Motor evoked potentials; Multiple sclerosis; Muscles; Nervous system; Neural networks; Neurophysiology; Peripheral blood; Pyramidal tracts; Transcranial magnetic stimulation
• ISSN: 2227-9059; 2227-9059
• DOI: 10.3390/biomedicines11102870

Expression of CD40 and CD192 markers in different monocyte subpopulations has been reported to be altered in people with MS (pwMS). Also, functional connectivity of the corticospinal motor system pathway alterations has been proved by transcranial magnetic stimulation (TMS). The study objective was to investigate the expression of CD40 and CD192 in classical (CD14 CD16 ), intermediate CD14 CD16 and non-classical (CD14 CD16 ) blood monocyte subpopulations in pwMS, undergoing neurophysiological TMS assessment of the corticospinal tract integrity by recording motor-evoked potentials (MEPs). Radiological examination on lesion detection with MRI was performed for 23 patients with relapsing-remitting MS treated with teriflunomide. Then, immunological analysis was conducted on peripheral blood samples collected from the patients and 10 healthy controls (HC). The blood samples were incubated with anti-human CD14, CD16, CD40 and CD192 antibodies. Next, pwMS underwent neurological testing of functional disability (EDSS) and TMS assessment with recording MEPs from upper and lower extremity muscles. The results show that in comparison to HC subjects, both pwMS with normal and altered MEP findings (prolonged MEP latency or absent MEP response) had significantly decreased surface receptor expression measured (MFIs) of CD192 and increased CD40 MFI in classical monocytes, and significantly increased percentages of classical and total monocytes positive for CD40. Knowing CD40's pro-inflammatory action, and CD192 as a molecule that enables the passing of monocytes into the brain, decreased CD192 in classical monocytes could represent a beneficial anti-inflammatory parameter.