Plant Gel-Mediated Synthesis of Gold-Coated Nanoceria Using Ferula gummosa : Characterization and Estimation of Its Cellular Toxicity toward Breast Cancer Cell Lines

• Published in: Journal of functional biomaterials 2023-06, Vol.14 (7), p.332
• Main Authors: Mousavi-Kouhi, Seyed Mousa, Beyk-Khormizi, Abdollah, Amiri, Mohammad Sadegh, Mashreghi, Mohammad, Hashemzadeh, Alireza, Mohammadzadeh, Vahideh, Alavi, Fariba, Mottaghipisheh, Javad, Sarafraz Ardakani, Mohammad Reza, Taghavizadeh Yazdi, Mohammad Ehsan
• Format: Article
• Language: English
• Subjects: Biomaterials Science; Biomaterialvetenskap; Breast cancer; Cancer; Cancer therapies; Cell death; cell toxicity; Coatings; Communication; Crystallites; Crystals; Cytotoxicity; Dispersion; Displays; Energy dispersive X ray spectroscopy; Ferula; Ferula gummosa; Field emission microscopy; Fourier transforms; Gold; gold coating; Gold coatings; green synthesis; Infrared spectroscopy; Light scattering; Microscopy; nanoceria; Nanocomposites; Nanoparticles; Oxidative stress; Particle size; Photon correlation spectroscopy; Room temperature; Scanning electron microscopy; Spectrum analysis; Toxicity; Toxicology; Tumor cell lines; X ray powder diffraction; X-ray spectroscopy; Zeta potential
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb14070332

In this study, a novel method using gums as a capping agent was used to synthesize the nanoceria for the first time. The method was economical and performed at room temperature. Furthermore, it was coated with gold (Au/nanoceria) and fully characterized using X-ray powder diffraction (XRD), field emission scanning electron microscopy with energy-dispersive X-ray spectroscopy (FESEM-EDX), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and zeta potential (ζ potential). The crystallite size obtained from the results was 28.09 nm for Au/nanoceria. The energy-dispersive X-ray spectroscopy (EDX) analysis of Au/nanoceria revealed the compositional constituents of the product, which display the purity of the Au/nanoceria. The cell toxicity properties of the non-doped and Au-coated nanoceria were identified by a MTT analysis on a breast cancer cell line (MCF7). Additionally, human foreskin fibroblast cells (HFF) were used as a normal cell line. The cytotoxicity results indicated that the toxicological effect of Au/nanoceria on cancer cells was significant while having little toxic effect on normal cells. The toxicity effect of nanoceria clearly shows the dependence on dose and time, so, with increasing the dose of Au/nanoceria, the death of cancer cells also increases.