Microbial enhance of chitosan production by Rhizopus arrhizus using agroindustrial substrates

This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Par...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2012-04, Vol.17 (5), p.4904-4914
Main Authors: Cardoso, Antonio, Lins, Clarissa Izabel M, dos Santos, Ednaldo Ramos, Freitas Silva, Marta C, Campos-Takaki, Galba M
Format: Article
Language:English
Subjects:
agroindustrial substrates
Biomass
biopolymer
Biopolymers
Carbon
chitosan
Chitosan - metabolism
Crops, Agricultural - metabolism
Crystallization
Fermentation
Fungi
Honey
Liquor
Microscopy
Morphology
Nitrogen
Rhizopus - metabolism
Rhizopus - ultrastructure
Rhizopus arrhizus
Spectroscopy, Fourier Transform Infrared
X-Ray Diffraction
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Summary:This study investigated the potential of Rhizopus arrhizus UCP 402 for producing chitosan using corn steep liquor and honey as agroindustrial nitrogen and carbon sources. A complete factorial design was used to assess the improved biomass and chitosan production. The results were evaluated using Pareto charts (Statistica 7.0 software). The chitosan obtained was characterized by X-ray diffraction. The cristallinity index (I(C)), and infrared spectroscopy (FTIR) were used to evaluate the degree of deacetylation (DD %). The morphological aspects of the R. arrhizus were evaluated by measuring the diameter of the colonies by light microscopy. The results obtained showed higher biomass and chitosan yields (20.61 g/L and 29.3 mg/g), respectively, in the selected assays. The characterization of the macromolecular arrangement of chitosan showed a crystallinity index compatible with the literature, and the infrared peaks confirmed a degree of 86%. The experimental data obtained suggest that adding honey to corn steep liquor is a promising way to improve microbiological chitosan production.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules17054904