PHLPP1 inhibits the growth and aerobic glycolysis activity of human ovarian granular cells through inactivating AKT pathway

Polycystic ovary syndrome (PCOS) is a disorder characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphologic features, and PCOS is associated with infertility. PH domain Leucine-rich repeat Protein Phosphatase 1 (PHLPP1) has been shown to regulate AKT. The aim of prese...

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Published in:BMC women's health 2024-01, Vol.24 (1), p.25-25, Article 25
Main Authors: Yang, Xiaoyan, A, Min, Gegen, Tana, Daoerji, Badema, Zheng, Yue, Wang, Aiming
Format: Article
Language:English
Subjects:
Acidification
AKT signaling
Analysis
Apoptosis
Care and treatment
Cell growth
Diagnosis
Enzymes
Female
Glucose metabolism
Glycolysis
Health aspects
Hormones, Sex
Humans
Infertility
Kinases
Measurement
Metabolism
Motility
Nuclear Proteins
Ovaries
PHLPP1
Phosphatases
Phosphoprotein Phosphatases - genetics
Polycystic Ovary Syndrome
Proteins
Proto-Oncogene Proteins c-akt
Reactive Oxygen Species
Sperm
Stein-Leventhal syndrome
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creator Yang, Xiaoyan
A, Min
Gegen, Tana
Daoerji, Badema
Zheng, Yue
Wang, Aiming
description Polycystic ovary syndrome (PCOS) is a disorder characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphologic features, and PCOS is associated with infertility. PH domain Leucine-rich repeat Protein Phosphatase 1 (PHLPP1) has been shown to regulate AKT. The aim of present study is to investigate the role of PHLPP1 in PCOS. The expression levels of PHLPP1 in dihydrotestosterone (DHT)-treated human ovarian granular KGN cells were determined by qRT-PCR and Western blot. PHLPP1 was silenced or overexpressed using lentivirus. Cell proliferation was detected by CCK-8. Apoptosis and ROS generation were analyzed by flow cytometry. Glycolysis was analyzed by measuring extracellular acidification rate (ECAR). DHT treatment suppressed proliferation, promoted apoptosis, enhanced ROS, and inhibited glycolysis in KGN cells. PHLPP1 silencing alleviated the DHT-induced suppression of proliferation and glycolysis, and promotion of apoptosis and ROS in KGN cells. PHLPP1 regulated cell proliferation and glycolysis in human KGN cells via the AKT signaling pathway. Our results showed that PHLPP1 mediates the proliferation and aerobic glycolysis activity of human ovarian granular cells through regulating AKT signaling.
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PH domain Leucine-rich repeat Protein Phosphatase 1 (PHLPP1) has been shown to regulate AKT. The aim of present study is to investigate the role of PHLPP1 in PCOS. The expression levels of PHLPP1 in dihydrotestosterone (DHT)-treated human ovarian granular KGN cells were determined by qRT-PCR and Western blot. PHLPP1 was silenced or overexpressed using lentivirus. Cell proliferation was detected by CCK-8. Apoptosis and ROS generation were analyzed by flow cytometry. Glycolysis was analyzed by measuring extracellular acidification rate (ECAR). DHT treatment suppressed proliferation, promoted apoptosis, enhanced ROS, and inhibited glycolysis in KGN cells. PHLPP1 silencing alleviated the DHT-induced suppression of proliferation and glycolysis, and promotion of apoptosis and ROS in KGN cells. PHLPP1 regulated cell proliferation and glycolysis in human KGN cells via the AKT signaling pathway. Our results showed that PHLPP1 mediates the proliferation and aerobic glycolysis activity of human ovarian granular cells through regulating AKT signaling.</description><identifier>ISSN: 1472-6874</identifier><identifier>EISSN: 1472-6874</identifier><identifier>DOI: 10.1186/s12905-023-02872-5</identifier><identifier>PMID: 38184561</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Acidification ; AKT signaling ; Analysis ; Apoptosis ; Care and treatment ; Cell growth ; Diagnosis ; Enzymes ; Female ; Glucose metabolism ; Glycolysis ; Health aspects ; Hormones, Sex ; Humans ; Infertility ; Kinases ; Measurement ; Metabolism ; Motility ; Nuclear Proteins ; Ovaries ; PHLPP1 ; Phosphatases ; Phosphoprotein Phosphatases - genetics ; Polycystic Ovary Syndrome ; Proteins ; Proto-Oncogene Proteins c-akt ; Reactive Oxygen Species ; Sperm ; Stein-Leventhal syndrome</subject><ispartof>BMC women's health, 2024-01, Vol.24 (1), p.25-25, Article 25</ispartof><rights>2024. 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PH domain Leucine-rich repeat Protein Phosphatase 1 (PHLPP1) has been shown to regulate AKT. The aim of present study is to investigate the role of PHLPP1 in PCOS. The expression levels of PHLPP1 in dihydrotestosterone (DHT)-treated human ovarian granular KGN cells were determined by qRT-PCR and Western blot. PHLPP1 was silenced or overexpressed using lentivirus. Cell proliferation was detected by CCK-8. Apoptosis and ROS generation were analyzed by flow cytometry. Glycolysis was analyzed by measuring extracellular acidification rate (ECAR). DHT treatment suppressed proliferation, promoted apoptosis, enhanced ROS, and inhibited glycolysis in KGN cells. PHLPP1 silencing alleviated the DHT-induced suppression of proliferation and glycolysis, and promotion of apoptosis and ROS in KGN cells. PHLPP1 regulated cell proliferation and glycolysis in human KGN cells via the AKT signaling pathway. 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PH domain Leucine-rich repeat Protein Phosphatase 1 (PHLPP1) has been shown to regulate AKT. The aim of present study is to investigate the role of PHLPP1 in PCOS. The expression levels of PHLPP1 in dihydrotestosterone (DHT)-treated human ovarian granular KGN cells were determined by qRT-PCR and Western blot. PHLPP1 was silenced or overexpressed using lentivirus. Cell proliferation was detected by CCK-8. Apoptosis and ROS generation were analyzed by flow cytometry. Glycolysis was analyzed by measuring extracellular acidification rate (ECAR). DHT treatment suppressed proliferation, promoted apoptosis, enhanced ROS, and inhibited glycolysis in KGN cells. PHLPP1 silencing alleviated the DHT-induced suppression of proliferation and glycolysis, and promotion of apoptosis and ROS in KGN cells. PHLPP1 regulated cell proliferation and glycolysis in human KGN cells via the AKT signaling pathway. 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subjects Acidification
AKT signaling
Analysis
Apoptosis
Care and treatment
Cell growth
Diagnosis
Enzymes
Female
Glucose metabolism
Glycolysis
Health aspects
Hormones, Sex
Humans
Infertility
Kinases
Measurement
Metabolism
Motility
Nuclear Proteins
Ovaries
PHLPP1
Phosphatases
Phosphoprotein Phosphatases - genetics
Polycystic Ovary Syndrome
Proteins
Proto-Oncogene Proteins c-akt
Reactive Oxygen Species
Sperm
Stein-Leventhal syndrome
title PHLPP1 inhibits the growth and aerobic glycolysis activity of human ovarian granular cells through inactivating AKT pathway
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