Building synthetic chromosomes from natural DNA

De novo chromosome synthesis is costly and time-consuming, limiting its use in research and biotechnology. Building synthetic chromosomes from natural components is an unexplored alternative with many potential applications. In this paper, we report CReATiNG ( Cl oning, Re programming, and A ssembli...

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Bibliographic Details
Published in:Nature communications 2023-12, Vol.14 (1), p.8337-8337, Article 8337
Main Authors: Coradini, Alessandro L. V., Ville, Christopher Ne, Krieger, Zachary A., Roemer, Joshua, Hull, Cara, Yang, Shawn, Lusk, Daniel T., Ehrenreich, Ian M.
Format: Article
Language:English
Subjects:
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Artificial chromosomes
Biotechnology
Chromosome deletion
Chromosomes
Chromosomes - genetics
Cloning
Deoxyribonucleic acid
DNA
DNA - genetics
DNA biosynthesis
Humanities and Social Sciences
multidisciplinary
Saccharomyces cerevisiae - genetics
Science
Science (multidisciplinary)
Synthesis
Synthetic Biology
Yeast
Yeasts
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Summary:De novo chromosome synthesis is costly and time-consuming, limiting its use in research and biotechnology. Building synthetic chromosomes from natural components is an unexplored alternative with many potential applications. In this paper, we report CReATiNG ( Cl oning, Re programming, and A ssembling Ti led N atural G enomic DNA), a method for constructing synthetic chromosomes from natural components in yeast. CReATiNG entails cloning segments of natural chromosomes and then programmably assembling them into synthetic chromosomes that can replace the native chromosomes in cells. We use CReATiNG to synthetically recombine chromosomes between strains and species, to modify chromosome structure, and to delete many linked, non-adjacent regions totaling 39% of a chromosome. The multiplex deletion experiment reveals that CReATiNG also enables recovery from flaws in synthetic chromosome design via recombination between a synthetic chromosome and its native counterpart. CReATiNG facilitates the application of chromosome synthesis to diverse biological problems. Building synthetic chromosomes from natural components is an unexplored alternative to de novo chromosome synthesis that may have many potential applications. In this paper, the authors report CReATiNG, a method for constructing synthetic chromosomes from natural components in yeast.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-023-44112-2