Effect of supplementing lysins and methionine to low-protein diets on growth performance, hepatic antioxidant capacity, immune status, and glycolytic activity of tibetan sheep

• Published in: BMC genomics 2024-06, Vol.25 (1), p.557-557
• Main Authors: Ji, Qiurong, Zhang, Fengshuo, Su, Quyangangmao, He, Tingli, Wu, Zhenling, Zhu, Kaina, Chen, Xuan, Wang, Zhiyou, Hou, Shengzhen, Gui, Linsheng
• Format: Article
• Language: English
• Subjects: Air quality management; Amino acid; Amino acids; Analysis; Animal Feed - analysis; Animals; Antioxidants; Antioxidants - metabolism; Body weight; Catalase; Correlation analysis; Dehydrogenases; Diet; Diet, Protein-Restricted - veterinary; Dietary Supplements; Differentially expressed genes; Diseases; DNA methylation; Dry matter; Enrichment; Enzymes; Feed additives; Feeding trials; Food and nutrition; Gene expression; Genes; Genetic aspects; Genomes; Glutathione; Glutathione peroxidase; Glycogen; Glycogens; Glycolysis; Glycolysis - drug effects; Growth; Health aspects; Hepatocytes; Immune response; Immune status; Immune system; Indicators; JunB protein; L-Lactate dehydrogenase; Lactate dehydrogenase; Liver; Liver - drug effects; Liver - metabolism; Low protein diet; Lysine - metabolism; Lysins; Male; Methionine; Methionine - administration & dosage; Methionine - metabolism; Methionine - pharmacology; Nutrient deficiency; Oxidative stress; Oxidoreductase; Peroxidase; Phenotypes; Physiology; Protein synthesis; Proteins; Sheep; Software; Tibetan lamb; Transcriptomes; Transcriptomic analysis
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-024-10480-2

Reducing the levels of dietary protein is an effective nutritional approach in lowering feed cost and nitrogen emissions in ruminants. The purpose of this study was to evaluate the effects of dietary Lys/Met ratio in a low protein diet (10%, dry matter basis) on the growth performance and hepatic function (antioxidant capacity, immune status, and glycolytic activity) in Tibetan lambs. Ninety two-month-old rams with an average weight of 15.37 ± 0.92 kg were randomly assigned to LP-L (dietary Lys/Met = 1:1), LP-M (dietary Lys/Met = 2:1) and LP-H (dietary Lys/Met = 3:1) treatments. The trial was conducted over 100 d, including 10 d of adaption to the diets. Hepatic phenotypes, antioxidant capacity, immune status, glycolytic activity and gene expression profiling was detected after the conclusion of the feeding trials. The results showed that the body weight was higher in the LP-L group when compared to those on the LP-M group (P