SP1-mediated up-regulation of lncRNA TUG1 underlines an oncogenic property in colorectal cancer

• Published in: Cell death & disease 2022-05, Vol.13 (5), p.433-10, Article 433
• Main Authors: Liu, Wei, Meng, Jin, Su, Rongjun, Shen, Changjun, Zhang, Shuai, Zhao, Yantao, Liu, Wenqi, Du, Jiang, Zhu, Shuai, Li, Pan, Wang, Zhigang, Li, Xiaoxia
• Format: Article
• Language: English
• Subjects: 13; 631/67; 692/699; Antibodies; Biochemistry; Biomedical and Life Sciences; Carcinogenesis - genetics; Cell Biology; Cell Culture; Cell Line, Tumor; Cell Proliferation - genetics; Colorectal cancer; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; F-Box Proteins - metabolism; Gene Expression Regulation, Neoplastic - genetics; Humans; Immunology; Jumonji Domain-Containing Histone Demethylases - metabolism; Life Sciences; MicroRNAs - genetics; MicroRNAs - metabolism; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Sp1 Transcription Factor - genetics; Sp1 Transcription Factor - metabolism; Transcription factors; Transcription Factors - metabolism; Up-Regulation - genetics
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-022-04805-w

The long non-coding RNA (lncRNA) taurine up-regulated gene 1 (TUG1) acts as tumor-promoting factor in colorectal cancer (CRC). We aimed to elucidate the mechanism by which the transcription factor specificity protein 1 (SP1) regulates TUG1 and microRNAs (miRs)/mRNAs in the context of CRC, which has not been fully studied before. Expression patterns of TUG1 and SP1 were determined in clinical CRC samples and cells, followed by identification of their interaction. Next, the functional significance of TUG1 in CRC was investigated. An in vivo CRC model was established to validate the effect of TUG1. The results demonstrated that TUG1 and SP1 were highly-expressed in CRC, wherein SP1 bound to the TUG1 promoter and consequently, positively regulated its expression. Silencing of TUG1 caused suppression of CRC cell growth and promotion of cell apoptosis. TUG1 could bind to miR-421 to increase KDM2A expression, a target gene of miR-421. TUG1 could activate the ERK pathway by impairing miR-421-targeted inhibition of KDM2A. Additionally, SP1 could facilitate the tumorigenesis of CRC cells in vivo by regulating the TUG1/miR-421/KDM2A/ERK axis. Altogether, the current study emphasizes the oncogenic role of TUG1 in CRC, and illustrates its interactions with the upstream transcription factor SP1 and the downstream modulatory axis miR-421/KDM2A/ERK, thus offering novel insights into the cancerogenic mechanism in CRC.