An experimental study on the Chinese lung adenocarcinoma cell clone CPA-Yang1-BR with brain metastasis potency in nude mice and in vivo imaging research

Lung cancer is the leading cause of cancer-related death in men and women. It is also the most common cause of brain metastases. A brain metastasis model is difficult to be established because of the presence of the blood-brain barrier (BBB) and the lack of optimal methods for detecting brain metast...

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Bibliographic Details
Published in:Zhongguo fei ai za zhi 2013-08, Vol.16 (8), p.391-399
Main Authors: Lei, Bei, Cao, Jie, Shen, Jie, Zhao, Lanxiang, Liang, Sheng, Meng, Qinggang, Xie, Wenhui, Yang, Shunfang
Format: Article
Language:Chinese
Subjects:
Adenocarcinoma - diagnosis
Adenocarcinoma - diagnostic imaging
Adenocarcinoma - pathology
Adenocarcinoma of Lung
Animal model
Animals
Blood-brain barrier
Brain
Brain Neoplasms - secondary
Breast cancer
Cell Proliferation
Cell Transformation, Neoplastic
Chemotherapy
Clone Cells - pathology
Cloning
Diagnostic Imaging
Disease Models, Animal
Hospitals
Humans
Lung cancer
Lung neoplasms
Lung Neoplasms - diagnosis
Lung Neoplasms - diagnostic imaging
Lung Neoplasms - pathology
Magnetic Resonance Imaging
Magnetic resonance imaging (MRI)
Male
Melanoma
Metastasis
Mice
Mice, Nude
Nuclear medicine
Positron emission tomography (PET)
Positron-Emission Tomography
Tomography, X-Ray Computed
Tumor metastasis
Tumors
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Summary:Lung cancer is the leading cause of cancer-related death in men and women. It is also the most common cause of brain metastases. A brain metastasis model is difficult to be established because of the presence of the blood-brain barrier (BBB) and the lack of optimal methods for detecting brain metastasis in nude mice. Thus, the establishment of a Chinese lung adenocarcinoma cell line and its animal model with brain metastasis potency and in vivo research is of great significance. CPA-Yang1 cells were obtained from a patient with human lung adenocarcinoma by lentiviral vector-mediated transfection of green fluorescence protein. Intracardiac inoculation of the cells was performed in nude mice, and brain metastatic lesions were detected using micro ¹⁸F FDG-PET/CT scanners, small animal in vivo imaging system for fluorescence, radionuclide and X ray fused imaging, magnetic resonance imaging (MRI) with sense body detection, and resection. The samples were divided into two parts for cell culture and histological dia
ISSN:1009-3419
1999-6187
DOI:10.3779/j.issn.1009-3419.2013.08.01