Alston Virus, a Novel Paramyxovirus Isolated from Bats Causes Upper Respiratory Tract Infection in Experimentally Challenged Ferrets

• Published in: Viruses 2018-11, Vol.10 (12), p.675
• Main Authors: Johnson, Rebecca I, Tachedjian, Mary, Rowe, Brenton, Clayton, Bronwyn A, Layton, Rachel, Bergfeld, Jemma, Wang, Lin-Fa, Marsh, Glenn A
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animal Diseases - immunology; Animal Diseases - virology; Animals; Antibiotics; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; bat-borne; Cell culture; Cell Line; Chiroptera; Chiroptera - virology; Disease Models, Animal; DNA polymerase; Female; Ferrets; Genome, Viral; Genomes; Glycoproteins; Infections; Neutralization Tests; New South Wales; Olfactory bulb; Parainfluenza; paramyxovirus; Phylogeny; Polymerase chain reaction; Proteins; Respiratory tract diseases; Respiratory tract infection; RNA polymerase; RNA, Viral; Rubulavirus - immunology; Rubulavirus - isolation & purification; Rubulavirus Infections - veterinary; Secretions; Urine; Viruses; Whole Genome Sequencing; Zoonoses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v10120675

Multiple viruses with zoonotic potential have been isolated from bats globally. Here we describe the isolation and characterization of a novel paramyxovirus, Alston virus (AlsPV), isolated from urine collected from an Australian pteropid bat colony in Alstonville, New South Wales. Characterization of AlsPV by whole-genome sequencing and analyzing antigenic relatedness revealed it is a rubulavirus that is closely related to parainfluenza virus 5 (PIV5). Intranasal exposure of mice to AlsPV resulted in no clinical signs of disease, although viral RNA was detected in the olfactory bulbs of two mice at 21 days post exposure. Oronasal challenge of ferrets resulted in subclinical upper respiratory tract infection, viral shedding in respiratory secretions, and detection of viral antigen in the olfactory bulb of the brain. These results imply that AlsPV may be similar to PIV5 in its ability to infect multiple mammalian host species. This isolation of a novel paramyxovirus with the potential to transmit from bats to other mammalian species reinforces the importance of continued surveillance of bats as a source of emerging viruses.