Lipase production by Yarrowia lipolytica using olive oil processing wastes as substrates

In this study solid and liquid wastes from the olive oil processing industry were evaluated as substrates for Yarrowia lipolytica growth with the aim of lipase production. Olive mill wastewater and olive oil cake seemed to provide necessary nutrients and physical support for the yeast to grow and pr...

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Bibliographic Details
Published in:Journal of the Serbian Chemical Society 2013, Vol.78 (6), p.781-794
Main Authors: Moftah, Omar, Grbavcic, Sanja, Moftah, Walid, Lukovic, Nevena, Prodanovic, Olivera, Jakovetic, Sonja, Knezevic-Jugovic, Zorica
Format: Article
Language:English
Subjects:
Cultivation
Fermentation
Lipase
lipase production
Liquid wastes
Nutrients
olive mill wastewater
Olive oil
olive oil cake
Processing industry
Solid state
solid state fermentation
Solids
Substrates
Wastewater
Yarrowia lipolytica
Yeast
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Summary:In this study solid and liquid wastes from the olive oil processing industry were evaluated as substrates for Yarrowia lipolytica growth with the aim of lipase production. Olive mill wastewater and olive oil cake seemed to provide necessary nutrients and physical support for the yeast to grow and produce enzyme. The highest lipolytic activity of 850 IU dm-3 was achieved after 4 days of submerged cultivation in supplemented olive mill wastewater. In addition, olive oil cake appeared to be a convenient substrate for lipase production under solid state fermentation mode. Lipase production was further improved by media supplementation and/or change in physical settings of the experiment. However, the most significant improvement of lipase production under solid state fermentation was achieved by an alkaline treatment of the substrate (more than 10-fold) when the amount of produced lipase reached up to ~40 IU g-1 of substrate.
ISSN:0352-5139
1820-7421
DOI:10.2298/JSC120905005M