Argonaute 2 restored erectile function and corpus cavernosum mitochondrial function by reducing apoptosis in a mouse model of cavernous nerve injury

• Published in: Investigative and clinical urology 2024-07, Vol.65 (4), p.400-410
• Main Authors: Huang, Yan, Yin, Guo Nan, Liu, Fang-Yuan, Fridayana, Fitri Rahma, Niloofar, Lashkari, Vo, Minh Nhat, Ryu, Ji-Kan
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis; argonaute proteins; Argonaute Proteins - genetics; Argonaute Proteins - metabolism; Disease Models, Animal; Erectile Dysfunction - etiology; Male; Mice; Mice, Inbred C57BL; Mitochondria - metabolism; mitochondria dysfunction; nerve regeneration; Original; Penile Erection - physiology; Penis - innervation; Peripheral Nerve Injuries - complications; reactive oxygen species
• ISSN: 2466-0493; 2466-054X; 2466-054X
• DOI: 10.4111/icu.20240077

To determine whether the overexpression of the Argonaute RNA-induced silencing complex catalytic component 2 (Ago2) improves erectile function in mice after cavernous nerve injury (CNI). Lentiviruses containing open reading frame (ORF) mouse clone (Ago2 O/E) were used to overexpress Ago2, and lentiviruses ORF negative control particles (NC) were used as a negative control. Three days before preparing the CNI model, we injected lentiviruses into the penises of 8-week-old male C57BL/6 mice. Animals were then divided into four groups: the sham operation control group and the CNI+phosphate-buffered saline, CNI+NC, and CNI+Ago2 O/E groups. One week later, erectile function was assessed by electrically stimulating cavernous nerves bilaterally and obtaining intracavernous pressure parameters. Penile tissue was also collected for molecular mechanism studies. Ago2 overexpression improved erectile function in mice after CNI-induced erectile dysfunction (ED). Immunofluorescence staining and Western blot analysis showed that under Ago2 overexpressing conditions, the contents of endothelial cells, pericytes, and neuronal cells increased in the penile tissues of CNI mice, and this was attributed to reduced apoptosis and ROS production. In addition, we also found that Ago2 overexpression could restore penile mitochondrial function, thereby improving erectile function in CNI-induced ED mice. Our findings demonstrate that Ago2 overexpression can reduce penile cell apoptosis, restore penile mitochondrial function, and improve erectile function in CNI-induced ED mice.