Investigation of the molecular mechanisms which contribute to the survival of the polychaete Platynereis spp. under ocean acidification conditions in the CO2 vent system of Ischia Island (Italy)

• Published in: Frontiers in Marine Science 2023-01, Vol.9
• Main Authors: Signorini, Silvia Giorgia, Munari, Marco, Cannavacciuolo, Antonio, Nannini, Matteo, Dolfini, Diletta, Chiarore, Antonia, Farè, Fiorenza, Fontana, Manuela, Caruso, Donatella, Gambi, Maria Cristina, Della Torre, Camilla
• Format: Article
• Language: English
• Subjects: Acclimatization; Acetylation; Acidification; Adaptation; Anthropogenic factors; Antioxidants; Biodiversity; Carbon dioxide; Climate change; CO2 vents; DNA methylation; Ecosystem biology; Emissions; Epigenetics; Experiments; Gene expression; histone modifications; Histones; Invertebrates; Laboratories; Lipids; Metabolic pathways; Metabolism; Metabolites; Metabolomics; Methylation; Molecular modelling; Ocean acidification; Organisms; Physiology; Platynereis; Polychaeta; polychaetes; Sterols; Survival
• ISSN: 2296-7745; 2296-7745
• DOI: 10.3389/fmars.2022.1067900

The continuous increase of CO 2 emissions in the atmosphere due to anthropogenic activities is one of the most important factors that contribute to Climate Change and generates the phenomenon known as Ocean Acidification (OA). Research conducted at the CO 2 vents of Castello Aragonese (Ischia, Italy), which represents a natural laboratory for the study of OA, demonstrated that some organisms, such as polychaetes, thrive under acidified conditions through different adaptation mechanisms. Some functional and ecological traits promoting tolerance to acidification in these organisms have been identified, while the molecular and physiological mechanisms underlying acclimatisation or genetic adaptation are still largely unknown. Therefore, in this study we investigated epigenetic traits, as histone acetylation and methylation, in Platynereis spp. individuals coming from the Castello vent, and from a nearby control site, in two different periods of the year (November-June). Untargeted metabolomics analysis was also carried out in specimens from the two sites. We found a different profile of acetylation of H2B histone in the control site compared to the vent as a function of the sampling period. Metabolomic analysis showed clear separation in the pattern of metabolites in polychaetes from the control site with respect to those from the Castello vent. Specifically, a significant reduction of lipid/sterols and nucleosides was measured in polychaetes from the vent. Overall results contribute to better understand the potential metabolic pathways involved in the tolerance to OA.