Carbon Nanoparticles Extracted from Date Palm Fronds for Fluorescence Bioimaging: In Vitro Study

• Published in: Journal of functional biomaterials 2022-11, Vol.13 (4), p.218
• Main Authors: Mohiuddin, Shaik Muhammad U G, Saeed, Abdu, Alshahrie, Ahmed, Memić, Adnan, Aljoud, Fadwa, Abdullahi, Shittu, Organji, Hussam A, Salah, Numan
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Biocompatibility; bioimaging; Biomass; Biomedical engineering; Biomedical materials; Boron nitride; Breast cancer; Carbon; carbon nanoparticles (CNPs); Cell culture; Cell growth; Cell viability; Chemical elements; Colon cancer; Cytotoxicity; date palm; Emissions; Ethylenediaminetetraacetic acid; Fluorescence; Fronds; Medical imaging; Morphology; Nanoparticles; Nanotechnology; Photoluminescence; Radiation; Regenerative medicine; Scanning devices; Semiconductors; Spectrum analysis; Toxicity; Tumor cell lines
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb13040218

Numerous studies have been reported on single- and multicolored highly fluorescent carbon nanoparticles (FCNPs) originating from various sources and their potential applications in bioimaging. Herein, multicolored biocompatible carbon nanoparticles (CNPs) unsheathed from date palm fronds were studied. The extracted CNPs were characterized via several microscopic and spectroscopic techniques. The results revealed that the CNPs were crystalline graphitic and hydrophilic in nature with sizes ranging from 4 to 20 nm. The unsheathed CNPs showed exemplary photoluminescent (PL) properties. They also emitted bright blue colors when exposed to ultraviolet (UV) light. Furthermore, in vitro cellular uptake and cell viability in the presence of CNPs were also investigated. The cell viability of human colon cancer (HCT-116) and breast adenocarcinoma (MCF-7) cell lines with aqueous CNPs at different concentrations was assessed by a cell metabolic activity assay (MTT) for 24 and 48 h incubations. The results were combined to generate dose-response curves for the CNPs and evaluate the severity of their toxicity. The CNPs showed adequate fluorescence with high cell viability for in vitro cell imaging. Under the laser-scanning confocal microscope, the CNPs with HCT-116 and MCF-7 cell lines showed multicolor fluorescence emissions, including blue, green, and red colors when excited at 405, 458, and 561 nm, respectively. These results prove that unsheathed CNPs from date palm fronds can be used in diverse biomedical applications because of their low cytotoxicity, adequate fluorescence, eco-friendly nature, and cheap production.