Efficient Conversion of Acetate to 3-Hydroxypropionic Acid by Engineered Escherichia coli

Acetate, which is an abundant carbon source, is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we initi...

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Bibliographic Details
Published in:Catalysts 2018-11, Vol.8 (11), p.525
Main Authors: Lee, Ji, Cha, Sanghak, Kang, Chae, Lee, Geon, Lim, Hyun, Jung, Gyoo
Format: Article
Language:English
Subjects:
3-hydroxypropionic acid
acetate
Biomass
Biosynthesis
Carbon
Catalysts
Cell growth
Chemical reactions
Conversion
E coli
Engineering
fatty acid synthesis
Fatty acids
Fermentation
Glycerol
metabolic engineering
microbial production
Microorganisms
Optimization
Raw materials
Reductases
Synthesis
synthetic biology
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Summary:Acetate, which is an abundant carbon source, is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid (3-HP) from acetate with engineered Escherichia coli. For the efficient conversion of acetate to 3-HP, we initially introduced heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate). The results indicated that acetate can be used as a promising carbon source for microbial processes and that 3-HP can be produced from acetate with a high yield (44.6% of the theoretical maximum yield).
ISSN:2073-4344
2073-4344
DOI:10.3390/catal8110525