The LiaSR Two-Component System Regulates Resistance to Chlorhexidine in Streptococcus mutans

Chlorhexidine (CHX) is widely considered to be the gold standard for preventing dental caries. However, it is possible to induce resistance to CHX. The LiaSR two-component system has been identified that contributed to CHX resistance in , which is one of the major pathogens in dental caries. However...

Full description

Saved in:
Bibliographic Details
Published in:Microorganisms (Basel) 2024-02, Vol.12 (3), p.468
Main Authors: Huang, Shan, Huang, Jing, Du, Jingyun, Li, Yijun, Wu, Minjing, Chen, Shuai, Zhan, Ling, Huang, Xiaojing
Format: Article
Language:English
Subjects:
Assaying
Bacteria
Biofilms
Cell membranes
cells envelope stress responses
Chlorhexidine
chlorhexidine resistance
Cytochrome c
Dental caries
Depolarization
Efflux
efflux pump
Hydrophobicity
LiaSR two-component system
Membrane potential
Mutants
Streptococcus mutans
Surface charge
Teeth
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chlorhexidine (CHX) is widely considered to be the gold standard for preventing dental caries. However, it is possible to induce resistance to CHX. The LiaSR two-component system has been identified that contributed to CHX resistance in , which is one of the major pathogens in dental caries. However, the underlying mechanisms remain unclear. In this study, an MIC assay and a viability assessment demonstrated that after deleting the and genes, the sensitivity of mutants could increase. The Nile Red efflux assay exhibited that the efflux rates of mutants were significantly decreased. The RT-qPCR results indicated that the LiaSR two-component system-mediating influence on the expression of in contributed to the efflux rate. The hydrophobicity assay and membrane potential assay showed that the mutants had higher levels of hydrophobicity and depolarization, suggesting that their membranes were more easily disturbed. The TEM graphs revealed that the border of the cell membrane was unclear in mutants compared with the wild-type strain, indicating that the cell envelope's stress response may have been inhibited. While the surface charge of mutants showed no significant difference in the wild-type strain according to the result of cytochrome c-based charged determination. This study provides valuable novel insights into the mechanisms of the LiaSR two-component system in the CHX resistance of .
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12030468