Root Growth and Enzymes Related to the Lignification of Maize Seedlings Exposed to the Allelochemical L-DOPA

L-3,4-Dihydroxyphenylalanine (L-DOPA) is a known allelochemical exuded from the roots of velvet bean (Mucuna pruriens L. Fabaceae). In the current work, we analyzed the effects of L-DOPA on the growth, the activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), and peroxidase...

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Bibliographic Details
Published in:TheScientificWorld 2013-01, Vol.2013 (2013), p.1-6
Main Authors: Ferrarese-Filho, O., Ferrarese, Maria de Lourdes Lucio, Parizotto, Angela Valderrama, Soares, Anderson Ricardo, Siqueira-Soares, Rita de Cássia
Format: Article
Language:English
Subjects:
Agricultural research
Ammonia
Cell walls
Corn
Dihydroxyphenylalanine
Enzymes
Exudation
Fabaceae
Flux density
Growth
Growth (Plants)
Growth chambers
Levodopa
Levodopa - chemistry
Levodopa - pharmacology
Lignin
Lignin - metabolism
Metabolism
Mucuna pruriens
Peroxidase
Phenylalanine
Phenylalanine ammonia-lyase
Physiology
Plant biology
Plant growth
Plant Roots - drug effects
Plant Roots - physiology
Proteins
Roots
Seedlings
Seedlings - drug effects
Seeds
Soybeans
Tyrosine
Weight
Zea mays
Zea mays - drug effects
Zea mays - physiology
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Summary:L-3,4-Dihydroxyphenylalanine (L-DOPA) is a known allelochemical exuded from the roots of velvet bean (Mucuna pruriens L. Fabaceae). In the current work, we analyzed the effects of L-DOPA on the growth, the activities of phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL), and peroxidase (POD), and the contents of phenylalanine, tyrosine, and lignin in maize (Zea mays) roots. Three-day-old seedlings were cultivated in nutrient solution with or without 0.1 to 2.0 mM L-DOPA in a growth chamber (25°C, light/dark photoperiod of 12/12, and photon flux density of 280 μmol m−2 s−1) for 24 h. The results revealed that the growth (length and weight) of the roots, the PAL, TAL, and soluble and cell wall-bound POD activities decreased, while phenylalanine, tyrosine, and lignin contents increased after L-DOPA exposure. Together, these findings showed the susceptibility of maize to L-DOPA. In brief, these results suggest that the inhibition of PAL and TAL can accumulate phenylalanine and tyrosine, which contribute to enhanced lignin deposition in the cell wall followed by a reduction of maize root growth.
ISSN:2356-6140
1537-744X
1537-744X
DOI:10.1155/2013/134237