Cholinesterase inhibitors isolated from bilberry fruit

•Bilberry fruit is confirmed to be a source of cholinesterase inhibitors.•The chemical structure of inhibitors was determined by HPLC, FT-IR, NMR and LC–MS.•The characterized compounds were derivatives of chlorogenic and benzoic acids. Cholinesterases (ChEs) are key enzymes in the pathogenesis of Al...

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Bibliographic Details
Published in:Journal of functional foods 2014-11, Vol.11, p.313-321
Main Authors: Borowiec, Kamila, Szwajgier, Dominik, Targoński, Zdzisław, Demchuk, Oleg M., Cybulska, Justyna, Czernecki, Tomasz, Malik, Agnieszka
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Alzheimer's disease
Bilberry
Butyrylcholinesterase
Inhibitor
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Summary:•Bilberry fruit is confirmed to be a source of cholinesterase inhibitors.•The chemical structure of inhibitors was determined by HPLC, FT-IR, NMR and LC–MS.•The characterized compounds were derivatives of chlorogenic and benzoic acids. Cholinesterases (ChEs) are key enzymes in the pathogenesis of Alzheimer's disease (AD). A growing body of evidence suggests that plants deliver compounds able to inhibit ChEs (e.g., huperzine A, galanthamine, and physostigmine), thus playing a beneficial therapeutic role in the treatment of AD. Screening for cholinesterase inhibitors (ChEIs) in selected fruits and vegetables showed that extract prepared from bilberry fruit effectively inhibited the activity of acetyl- and butyrylcholinesterase. The purification of ChEIs from bilberry fruit followed by HPLC-UV, FT-IR, NMR, and LC–MS demonstrated that the studied compounds were derivatives of chlorogenic and benzoic acids. These results confirm that bilberry fruit may serve as a useful source of ChEIs, leading to the attenuation of memory deficit caused by AD.
ISSN:1756-4646
2214-9414
DOI:10.1016/j.jff.2014.10.008