Identification of Candidate Genes Associated With Tolerance to Apple Replant Disease by Genome-Wide Transcriptome Analysis

Apple replant disease (ARD) is a worldwide economic risk in apple cultivation for fruit tree nurseries and fruit growers. Several studies on the reaction of apple plants to ARD are documented but less is known about the genetic mechanisms behind this symptomatology. RNA-seq analysis is a powerful to...

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Bibliographic Details
Published in:Frontiers in microbiology 2022-05, Vol.13, p.888908-888908
Main Authors: Reim, Stefanie, Winkelmann, Traud, Cestaro, Alessandro, Rohr, Annmarie-Deetja, Flachowsky, Henryk
Format: Article
Language:English
Subjects:
apple rootstocks
gibberellin biosynthesis
M9
Malus ×robusta 5
Microbiology
RNA-seq validation
soil-borne disease
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Summary:Apple replant disease (ARD) is a worldwide economic risk in apple cultivation for fruit tree nurseries and fruit growers. Several studies on the reaction of apple plants to ARD are documented but less is known about the genetic mechanisms behind this symptomatology. RNA-seq analysis is a powerful tool for revealing candidate genes that are involved in the molecular responses to biotic stresses in plants. The aim of our work was to find differentially expressed genes in response to ARD in For this, we compared transcriptome data of the rootstock 'M9' (susceptible) and the wild apple genotype × 5 (Mr5, tolerant) after cultivation in ARD soil and disinfected ARD soil, respectively. When comparing apple plantlets grown in ARD soil to those grown in disinfected ARD soil, 1,206 differentially expressed genes (DEGs) were identified based on a log2 fold change, (LFC) ≥ 1 for up- and ≤ -1 for downregulation (  
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2022.888908