Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells by WNT Switch Method

The differentiation of ESCs into cardiomyocytes in vitro is an excellent and reliable model system for studying normal cardiomyocyte development in mammals, modeling cardiac diseases, and for use in drug screening. Mouse ESC differentiation still provides relevant biological information about cardia...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2024-01, Vol.13 (2), p.132
Main Authors: Mensah, Isaiah K, Emerson, Martin L, Tan, Hern J, Gowher, Humaira
Format: Article
Language:English
Subjects:
Animals
Cardiomyocytes
Cell culture
Cell Differentiation
Cell research
Coronary artery disease
Drug Evaluation, Preclinical
Drug screening
Embryo cells
Ethanol
Eye Diseases, Hereditary
heart
Heart cells
Heart diseases
in vitro differentiation
Invoices
Kinases
Mammals
mesoderm
Methods
Mice
Mouse Embryonic Stem Cells
Myocytes, Cardiac
Physiological aspects
Retinal Degeneration
Stem cells
Vision Disorders
Wnt protein
Wnt proteins
WNT signaling
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Summary:The differentiation of ESCs into cardiomyocytes in vitro is an excellent and reliable model system for studying normal cardiomyocyte development in mammals, modeling cardiac diseases, and for use in drug screening. Mouse ESC differentiation still provides relevant biological information about cardiac development. However, the current methods for efficiently differentiating ESCs into cardiomyocytes are limiting. Here, we describe the "WNT Switch" method to efficiently commit mouse ESCs into cardiomyocytes using the small molecule WNT signaling modulators CHIR99021 and XAV939 in vitro. This method significantly improves the yield of beating cardiomyocytes, reduces number of treatments, and is less laborious.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells13020132