Chromatin-Remodelling ATPases ISWI and BRM Are Essential for Reproduction in the Destructive Pest Tuta absoluta

The tomato leaf miner ( ) is one of the top 20 plant pests worldwide. We cloned and identified the chromatin-remodelling ATPase genes and by RACE and bioinformatic analysis, respectively; used RT-qPCR to examine their expression patterns during different life cycle stages; and elucidated their roles...

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Bibliographic Details
Published in:International journal of molecular sciences 2022-03, Vol.23 (6), p.3267
Main Authors: Ji, Shun-Xia, Wu, Qiang-Wen, Bi, Si-Yan, Wang, Xiao-Di, Zhang, Gui-Fen, Wan, Fang-Hao, Lü, Zhi-Chuang, Liu, Wan-Xue
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Animals
bromo
Chromatin - genetics
Chromatin - metabolism
Chromatin remodeling
DNA, Complementary - metabolism
Double-stranded RNA
Eggs
Fecundity
Female
Females
Gene expression
Genes
Hatchability
imitation SWItch
Larva - metabolism
Lepidoptera - genetics
Lycopersicon esculentum - genetics
Moths - genetics
Pest control
Pests
Polypeptides
Proteins
Reproduction
RNA interference
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
RNA-mediated interference
Tuta absoluta
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Summary:The tomato leaf miner ( ) is one of the top 20 plant pests worldwide. We cloned and identified the chromatin-remodelling ATPase genes and by RACE and bioinformatic analysis, respectively; used RT-qPCR to examine their expression patterns during different life cycle stages; and elucidated their roles in insect reproduction using double-stranded RNA injections. The full-length cDNA of was 3428 bp and it encoded a 1025-aa polypeptide. The partial-length cDNA of was 3457 bp and it encoded a 1030-aa polypeptide. and were upregulated at the egg stage. Injection of or dsRNA at the late pupa stage significantly inhibited adult ovary development and reduced fecundity, hatchability, and longevity in the adult females. To the best of our knowledge, the present study was the first to perform molecular characterisations of two chromatin-remodelling ATPase genes and clarify their roles in fecundity. Chromatin-remodelling ATPases are potential RNAi targets for the control of and other insect pests. The present study was also the first to demonstrate the feasibility of reproductive inhibitory RNAi as a putative approach for the suppression of and other Lepidopteran insect populations.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23063267