The reference genome and transcriptome of the limestone langur, Trachypithecus leucocephalus, reveal expansion of genes related to alkali tolerance

• Published in: BMC biology 2021-04, Vol.19 (1), p.67-67, Article 67
• Main Authors: Que, Tengcheng, Wang, Huifeng, Yang, Weifei, Wu, Jianbao, Hou, Chenyang, Pei, Surui, Wu, Qunying, Li, Liu Ming, Wei, Shilu, Xie, Xing, Huang, Hongli, Chen, Panyu, Huang, Yiming, Wu, Aiqiong, He, Meihong, Nong, Dengpan, Wei, Xiao, Wu, Junyi, Nong, Ru, Huang, Ning, Zhou, Qingniao, Lin, Yaowang, Lu, Tingxi, Wei, Yongjie, Li, Shousheng, Yao, Jianglong, Zhong, Yanli, Qin, Huayong, Tan, Luohao, Li, Yingjiao, Li, Weidong, Liu, Tao, Liu, Sanyang, Yu, Yongyi, Qiu, Hong, Jiang, Yonghua, Li, Youcheng, Liu, Zhijin, Huang, Cheng Ming, Hu, Yanling
• Format: Article
• Language: English
• Subjects: Adaptation; Adaptation (Biology); Alkali tolerance; Analysis; Annotations; Assembly; Calcium; Calcium absorption; Calcium signalling; Chromosomes; Ecological effects; Ecological niches; Endangered animals; Endangered species; Gene expression; Gene families; Genes; Genetic aspects; Genome assembly; Genomes; Genomics; Homeostasis; Identification and classification; Karst; Limestone; Limestone karsts; Metabolism; Mineral ion binding; Mountains; Observations; Presbytis; Protection and preservation; Proteins; Trachypithecus leucocephalus; Transcriptomes
• ISSN: 1741-7007; 1741-7007
• DOI: 10.1186/s12915-021-00998-2

Trachypithecus leucocephalus, the white-headed langur, is a critically endangered primate that is endemic to the karst mountains in the southern Guangxi province of China. Studying the genomic and transcriptomic mechanisms underlying its local adaptation could help explain its persistence within a highly specialized ecological niche. In this study, we used PacBio sequencing and optical assembly and Hi-C analysis to create a high-quality de novo assembly of the T. leucocephalus genome. Annotation and functional enrichment revealed many genes involved in metabolism, transport, and homeostasis, and almost all of the positively selected genes were related to mineral ion binding. The transcriptomes of 12 tissues from three T. leucocephalus individuals showed that the great majority of genes involved in mineral absorption and calcium signaling were expressed, and their gene families were significantly expanded. For example, FTH1 primarily functions in iron storage and had 20 expanded copies. These results increase our understanding of the evolution of alkali tolerance and other traits necessary for the persistence of T. leucocephalus within an ecologically unique limestone karst environment.