Influences of sterol regulatory element binding protein-1c silencing on glucose production in HepG2 cells treated with free fatty acid

• Published in: Lipids in health and disease 2019-04, Vol.18 (1), p.89-89, Article 89
• Main Authors: Bai, Xiu-Ping, Dong, Feng, Yang, Guo-Hua, Zhang, Lei
• Format: Article
• Language: English
• Subjects: Apoptosis; Binding proteins; Care and treatment; Carnitine; Carnitine palmitoyltransferase; Desaturase; Diabetes; Ethanol; Fatty acid synthesis; Fatty acids; Fatty-acid synthase; Free fatty acid; Gene expression; Genes; Genetic aspects; Gluconeogenesis; Glucose; Glucose metabolism; Glucose production; Glucose-6-phosphatase; Hepatocytes; HepG2 cells; Influence; Insulin; Insulin resistance; Kinases; Lipids; Liver; Messenger RNA; Metabolism; Microscopy; Obesity; Oxidation; Palmitoyltransferase; Phosphatases; Phosphates; Phosphoenolpyruvate carboxylase; Protein binding; RNA; siRNA; SREBP-1c silencing; Stearoyl-CoA desaturase; Steroids (Organic compounds); Sterol regulatory element-binding protein; Sterols; Transcription factors; Type 2 diabetes
• ISSN: 1476-511X; 1476-511X
• DOI: 10.1186/s12944-019-1026-3

Elevation of exogenous free fatty acid (FFA) level leads to insulin resistance (IR) in liver, IR is manifested by elevated hepatic glucose production. We aim to study whether inhibition of endogenous fatty acid synthesis could decrease hepatic glucose production. Low-passage HepG2 cells derived from human liver tissue were cultured in medium supplemented with FFA to induce IR, the influences of sterol regulatory element binding protein-1c (SREBP-1c) silencing on glucose production of HepG2 cells were investigated, and genes responsible for fatty acid and glucose metabolism were detected by real-time PCR. Compared with HepG2 cells cultured in normal growth medium, glucose production of HepG2 cells treated by FFA was significantly increased {[(0.28 ± 0.01) vs (0.83 ± 0.02)] umol.ug protein, n = 6 wells, P