Guidelines for an optimized differential centrifugation of cells

Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose...

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Bibliographic Details
Published in:Biochemistry and biophysics reports 2023-12, Vol.36, p.101585-101585, Article 101585
Main Authors: Sharifian Gh, Mohammad, Norouzi, Fatemeh
Format: Article
Language:English
Subjects:
Cell
Centrifugation
Optimum sedimentation
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Summary:Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices.
ISSN:2405-5808
2405-5808
DOI:10.1016/j.bbrep.2023.101585