Guidelines for an optimized differential centrifugation of cells

Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose...

Full description

Saved in:
Bibliographic Details
Published in:Biochemistry and biophysics reports 2023-12, Vol.36, p.101585-101585, Article 101585
Main Authors: Sharifian Gh, Mohammad, Norouzi, Fatemeh
Format: Article
Language:English
Subjects:
Cell
Centrifugation
Optimum sedimentation
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c366t-795e8fa8fb07f71008741a1e2adfdf628a90f59ac77c697c4420b6af44ef94a73
container_end_page 101585
container_issue
container_start_page 101585
container_title Biochemistry and biophysics reports
container_volume 36
creator Sharifian Gh, Mohammad
Norouzi, Fatemeh
description Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices.
doi_str_mv 10.1016/j.bbrep.2023.101585
format article
fullrecord <record><control><sourceid>proquest_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_df59e5c4d84f42edb433e6fbccc238b7</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_df59e5c4d84f42edb433e6fbccc238b7</doaj_id><sourcerecordid>2902953371</sourcerecordid><originalsourceid>FETCH-LOGICAL-c366t-795e8fa8fb07f71008741a1e2adfdf628a90f59ac77c697c4420b6af44ef94a73</originalsourceid><addsrcrecordid>eNpNUU1rGzEUFKUlDq5_QaDssRe7-pb21hIaJ2DopT0LrfSekVlbrrR7SH59duMk5DSPYWYe7w0hN4xuGGX6x2HTdQXOG065mBll1SdyzSVVa2Wp_fxhXpBVrQdKJxG3iusrshCWGq01uyY_t2OK0KcT1AZzafypyechHdMTxCYmRChwGpLvmzBhSTju_ZDypMKJ6fv6lXxB31dYveKS_Lv7_ff2fr37s324_bVbB6H1sDatAoveYkcNGkapNZJ5BtxHjKi59S1F1fpgTNCtCVJy2mmPUgK20huxJA-X3Jj9wZ1LOvry6LJP7oXIZe98GVLowcUpCFSQ0UqUHGInhQCNXQiBC9vNWd8vWeeS_49QB3dMdb7GnyCP1fGW8lYJYdgkFRdpKLnWAvi-mlE3N-EO7qUJNzfhLk1Mrm-vC8buCPHd8_Z38Qz2j4Xz</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2902953371</pqid></control><display><type>article</type><title>Guidelines for an optimized differential centrifugation of cells</title><source>ScienceDirect</source><source>PubMed Central</source><creator>Sharifian Gh, Mohammad ; Norouzi, Fatemeh</creator><creatorcontrib>Sharifian Gh, Mohammad ; Norouzi, Fatemeh</creatorcontrib><description>Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices.</description><identifier>ISSN: 2405-5808</identifier><identifier>EISSN: 2405-5808</identifier><identifier>DOI: 10.1016/j.bbrep.2023.101585</identifier><identifier>PMID: 38076661</identifier><language>eng</language><publisher>Netherlands: Elsevier</publisher><subject>Cell ; Centrifugation ; Optimum sedimentation</subject><ispartof>Biochemistry and biophysics reports, 2023-12, Vol.36, p.101585-101585, Article 101585</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c366t-795e8fa8fb07f71008741a1e2adfdf628a90f59ac77c697c4420b6af44ef94a73</cites><orcidid>0000-0002-2332-0125 ; 0000-0003-3867-1611</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38076661$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharifian Gh, Mohammad</creatorcontrib><creatorcontrib>Norouzi, Fatemeh</creatorcontrib><title>Guidelines for an optimized differential centrifugation of cells</title><title>Biochemistry and biophysics reports</title><addtitle>Biochem Biophys Rep</addtitle><description>Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices.</description><subject>Cell</subject><subject>Centrifugation</subject><subject>Optimum sedimentation</subject><issn>2405-5808</issn><issn>2405-5808</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNpNUU1rGzEUFKUlDq5_QaDssRe7-pb21hIaJ2DopT0LrfSekVlbrrR7SH59duMk5DSPYWYe7w0hN4xuGGX6x2HTdQXOG065mBll1SdyzSVVa2Wp_fxhXpBVrQdKJxG3iusrshCWGq01uyY_t2OK0KcT1AZzafypyechHdMTxCYmRChwGpLvmzBhSTju_ZDypMKJ6fv6lXxB31dYveKS_Lv7_ff2fr37s324_bVbB6H1sDatAoveYkcNGkapNZJ5BtxHjKi59S1F1fpgTNCtCVJy2mmPUgK20huxJA-X3Jj9wZ1LOvry6LJP7oXIZe98GVLowcUpCFSQ0UqUHGInhQCNXQiBC9vNWd8vWeeS_49QB3dMdb7GnyCP1fGW8lYJYdgkFRdpKLnWAvi-mlE3N-EO7qUJNzfhLk1Mrm-vC8buCPHd8_Z38Qz2j4Xz</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>Sharifian Gh, Mohammad</creator><creator>Norouzi, Fatemeh</creator><general>Elsevier</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2332-0125</orcidid><orcidid>https://orcid.org/0000-0003-3867-1611</orcidid></search><sort><creationdate>202312</creationdate><title>Guidelines for an optimized differential centrifugation of cells</title><author>Sharifian Gh, Mohammad ; Norouzi, Fatemeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-795e8fa8fb07f71008741a1e2adfdf628a90f59ac77c697c4420b6af44ef94a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Cell</topic><topic>Centrifugation</topic><topic>Optimum sedimentation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharifian Gh, Mohammad</creatorcontrib><creatorcontrib>Norouzi, Fatemeh</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Directory of Open Access Journals</collection><jtitle>Biochemistry and biophysics reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharifian Gh, Mohammad</au><au>Norouzi, Fatemeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Guidelines for an optimized differential centrifugation of cells</atitle><jtitle>Biochemistry and biophysics reports</jtitle><addtitle>Biochem Biophys Rep</addtitle><date>2023-12</date><risdate>2023</risdate><volume>36</volume><spage>101585</spage><epage>101585</epage><pages>101585-101585</pages><artnum>101585</artnum><issn>2405-5808</issn><eissn>2405-5808</eissn><abstract>Literature reviews reveal a significant deficiency in conceptual comprehension concerning centrifugation, a crucial step in both medical and research protocols. The arbitrary fluctuations in centrifugal forces present a potential threat to the reproducibility of results. To address this, we propose concise guidelines that integrate key factors such as temperature, osmolarity, fluid volume, and viscosity. These guidelines aim to enhance comprehension of optimal sedimentation conditions for cell suspensions. Additionally, we introduce a standardized protocol for determining the optimal RCF and centrifugation time. The goal is to maximize sedimentation efficiency while minimizing cell damage, contributing to a universally applicable and reproducible method in centrifugation practices.</abstract><cop>Netherlands</cop><pub>Elsevier</pub><pmid>38076661</pmid><doi>10.1016/j.bbrep.2023.101585</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2332-0125</orcidid><orcidid>https://orcid.org/0000-0003-3867-1611</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2405-5808
ispartof Biochemistry and biophysics reports, 2023-12, Vol.36, p.101585-101585, Article 101585
issn 2405-5808
2405-5808
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_df59e5c4d84f42edb433e6fbccc238b7
source ScienceDirect; PubMed Central
subjects Cell
Centrifugation
Optimum sedimentation
title Guidelines for an optimized differential centrifugation of cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T10%3A55%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Guidelines%20for%20an%20optimized%20differential%20centrifugation%20of%20cells&rft.jtitle=Biochemistry%20and%20biophysics%20reports&rft.au=Sharifian%20Gh,%20Mohammad&rft.date=2023-12&rft.volume=36&rft.spage=101585&rft.epage=101585&rft.pages=101585-101585&rft.artnum=101585&rft.issn=2405-5808&rft.eissn=2405-5808&rft_id=info:doi/10.1016/j.bbrep.2023.101585&rft_dat=%3Cproquest_doaj_%3E2902953371%3C/proquest_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c366t-795e8fa8fb07f71008741a1e2adfdf628a90f59ac77c697c4420b6af44ef94a73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2902953371&rft_id=info:pmid/38076661&rfr_iscdi=true