Histone Octamer Structure Is Altered Early in ISW2 ATP-Dependent Nucleosome Remodeling

• Published in: Cell reports (Cambridge) 2019-07, Vol.28 (1), p.282-294.e6
• Main Authors: Hada, Arjan, Hota, Swetansu K., Luo, Jie, Lin, Yuan-chi, Kale, Seyit, Shaytan, Alexey K., Bhardwaj, Saurabh K., Persinger, Jim, Ranish, Jeff, Panchenko, Anna R., Bartholomew, Blaine
• Format: Article
• Language: English
• Subjects: chromatin remodeling; gene regulation; histones; ISWI; nucleosome dynamics; nucleosome movement; nucleosome structure; SANT domain; transcription
• ISSN: 2211-1247; 2211-1247
• DOI: 10.1016/j.celrep.2019.05.106

Nucleosomes are the fundamental building blocks of chromatin that regulate DNA access and are composed of histone octamers. ATP-dependent chromatin remodelers like ISW2 regulate chromatin access by translationally moving nucleosomes to different DNA regions. We find that histone octamers are more pliable than previously assumed and distorted by ISW2 early in remodeling before DNA enters nucleosomes and the ATPase motor moves processively on nucleosomal DNA. Uncoupling the ATPase activity of ISW2 from nucleosome movement with deletion of the SANT domain from the C terminus of the Isw2 catalytic subunit traps remodeling intermediates in which the histone octamer structure is changed. We find restricting histone movement by chemical crosslinking also traps remodeling intermediates resembling those seen early in ISW2 remodeling with loss of the SANT domain. Other evidence shows histone octamers are intrinsically prone to changing their conformation and can be distorted merely by H3-H4 tetramer disulfide crosslinking. [Display omitted] •DNA moves asynchronously across the histone octamer surface during ISW2 remodeling•Mutation of ISW2 or restricting histone movement uncouples DNA entering from exiting•The histone octamer structure distorts when DNA initially exits and before DNA enters•The histone octamer intrinsically distorts and can be stabilized by disulfide crosslinking Hada et al. show that ISW2 catalyzes DNA movement through nucleosomes with a short stretch of DNA persistently exiting the nucleosome before DNA enters nucleosomes. A consequence of asynchronous DNA movement is distortion of the histone octamer structure in which the rotational phasing of DNA is maintained on the octamer surface.