Evidence of Bioactive Compounds from Vernonia polyanthes Leaves with Topical Anti-Inflammatory Potential

• Published in: International journal of molecular sciences 2016-12, Vol.17 (12), p.1929-1929
• Main Authors: Rodrigues, Kamilla C M, Chibli, Lucas A, Santos, Bruna C S, Temponi, Vanessa S, Pinto, Nícolas C C, Scio, Elita, Del-Vechio-Vieira, Glauciemar, Alves, Maria S, Sousa, Orlando V
• Format: Article
• Language: English
• Subjects: ">d-glucosaminidase; Acetates - chemistry; Administration, Topical; Animals; Anti-Inflammatory Agents; Asteraceae; Brazil; Croton; ear edema; Herbal medicine; Hexanes - chemistry; Histopathology; Humans; Inflammation; Inflammation - drug therapy; Inflammation - pathology; Medicine, Traditional; Mice; myeloperoxidase; N-acetyl-β; Phytotherapy; Plant Extracts - administration & dosage; Plant Extracts - chemistry; Plant Leaves - chemistry; Skin - drug effects; Skin - pathology; Vernonia; Vernonia - chemistry; Vernonia polyanthes
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms17121929

Less. (Asteraceae), popularly known as "assa-peixe", is a plant species used in Brazilian traditional medicine for the treatment of cutaneous damage, cicatrization, inflammation, and rheumatism. Based on these ethnopharmacological findings, the current study evaluated the topical anti-inflammatory effects of the hexane (HEVP) and ethyl acetate (EAEVP) extracts from leaves in experimental models of skin inflammation. Chemical characterization was carried out by HPLC-UV/DAD analysis. Anti-inflammatory activity was evaluated using Croton oil-, arachidonic acid (AA)-, phenol-, ethyl phenylpropiolate (EPP)-, and capsaicin-induced ear edema models in mice. Histopathological evaluation and measurements of myeloperoxidase (MPO) and -acetyl-β-d-glucosaminidase (NAG) enzymes were also performed. Rutin, luteolin, and apigenin were identified in EAEVP. Topically applied HEVP and EAEVP significantly ( < 0.05, < 0.01 or < 0.001) reduced edema induced by five different irritants at the doses tested (0.1, 0.5 and 1.0 mg/ear). Histopathological analysis revealed a reduction of edema, inflammatory cell infiltration, and vasodilation. In addition, the enzymes activity (MPO and NAG) in the ear tissues was reduced by the topical treatment of HEVP and EAEVP ( < 0.05, < 0.01 or < 0.001). The results suggest that leaves are effective against cutaneous damage, which support its traditional use and open up new possibilities for the treatment of skin disorders.