The use of soft X-ray tomography to explore mitochondrial structure and function

Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three-dimensional whole cell mapping technique is required....

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Bibliographic Details
Published in:Molecular metabolism (Germany) 2022-03, Vol.57 (C), p.101421-101421, Article 101421
Main Authors: Loconte, Valentina, White, Kate L.
Format: Article
Language:English
Subjects:
Cell mapping
Mitochondria
Review
Spatial biology
Structural biology
Tomography
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Summary:Mitochondria are cellular organelles responsible for energy production, and dysregulation of the mitochondrial network is associated with many disease states. To fully characterize the mitochondrial network's structure and function, a three-dimensional whole cell mapping technique is required. This review highlights the use of soft X-ray tomography (SXT) as a relatively high-throughput approach to quantify mitochondrial structure and function under multiple cellular conditions. The use of SXT opens the door for mapping cellular rearrangements during critical processes such as insulin secretion, stem cell differentiation, or disease progression. SXT provides unique information such as biochemical compositions or molecular densities of organelles and allows for unbiased, label-free imaging of intact whole cells. Mapping mitochondria in the context of the near-native cellular environment will reveal more information regarding mitochondrial network functions within the cell. •Soft X-ray tomography (SXT) generates 3D organelle maps of intact cells.•3D maps reveal the positions of mitochondria and their molecular densities.•SXT can be used to quantify and compare organelle contacts between conditions.•SXT is unbiased imaging that identifies the contents of subcellular neighborhoods.•SXT provides an exciting path for exploring metabolic dysfunction.
ISSN:2212-8778
2212-8778
DOI:10.1016/j.molmet.2021.101421