Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of Treponema pallidum and Haemophilus ducreyi

Yaws, a neglected tropical disease caused by the bacterium Treponema pallidum subspecies pertenue, manifests as ulcerative skin lesions. Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to distinguish yaws from infections that cause similar sk...

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Bibliographic Details
Published in:Emerging infectious diseases 2020-02, Vol.26 (2), p.282-288
Main Authors: Becherer, Lisa, Knauf, Sascha, Marks, Michael, Lueert, Simone, Frischmann, Sieghard, Borst, Nadine, von Stetten, Felix, Bieb, Sibauk, Adu-Sarkodie, Yaw, Asiedu, Kingsley, Mitjà, Oriol, Bakheit, Mohammed
Format: Article
Language:English
Subjects:
and
Bacterial infections
Chancroid - diagnosis
Chancroid - microbiology
Child
Comparative analysis
Deoxyribonucleic acid
Disease eradication
Diseases
DNA
DNA polymerase
Female
Genetic research
Ghana
Haemophilus ducreyi
Haemophilus ducreyi - isolation & purification
Health aspects
Hemophilus infections
Humans
Infection
Laboratories
LAMP
loop-mediated isothermal amplification
Male
Medical tests
Molecular Diagnostic Techniques
Multiplex Mediator Displacement Loop-Mediated Isothermal Amplification for Detection of
neglected tropical disease
Novels
Nucleic Acid Amplification Techniques
Papua New Guinea
Patients
Polymerase chain reaction
Sensitivity and Specificity
Skin
Streptococcus infections
Surveillance
Treponema pallidum
Treponema pallidum - isolation & purification
Yaws
Yaws - diagnosis
Yaws - microbiology
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Summary:Yaws, a neglected tropical disease caused by the bacterium Treponema pallidum subspecies pertenue, manifests as ulcerative skin lesions. Nucleic acid amplification tests, like loop-mediated isothermal amplification (LAMP), are versatile tools to distinguish yaws from infections that cause similar skin lesions, primarily Haemophilus ducreyi. We developed a novel molecular test to simultaneously detect T. pallidum and H. ducreyi based on mediator displacement LAMP. We validated the T. pallidum and H. ducreyi LAMP (TPHD-LAMP) by testing 293 clinical samples from patients with yaws-like lesions. Compared with quantitative PCR, the TPHD-LAMP demonstrated high sensitivity and specificity for T. pallidum (84.7% sensitivity, 95.7% specificity) and H. ducreyi (91.6% sensitivity, 84.8% specificity). This novel assay provided rapid molecular confirmation of T. pallidum and H. ducreyi DNA and might be suitable for use at the point of care. TPHD-LAMP could support yaws eradication by improving access to molecular diagnostic tests at the district hospital level.
ISSN:1080-6040
1080-6059
DOI:10.3201/eid2602.190505