The Virome of 'Lamon Bean': Application of MinION Sequencing to Investigate the Virus Population Associated with Symptomatic Beans in the Lamon Area, Italy

• Published in: Plants (Basel) 2022-03, Vol.11 (6), p.779
• Main Authors: Tarquini, Giulia, Martini, Marta, Maestri, Simone, Firrao, Giuseppe, Ermacora, Paolo
• Format: Article
• Language: English
• Subjects: bean common mosaic virus; bean yellow mosaic virus; Beans; cucumber mosaic virus; Economics; Ecotypes; Genomes; peanut stunt virus; Peanuts; Phaseolus vulgaris; Phylogenetics; plant disease; Plant virus diseases; Plant viruses; Polymerase chain reaction; Profitability; Proteins; Sequences; Signs and symptoms; Viral infections; virology; Viruses
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants11060779

'Lamon bean' is a protected geographical indication (PGI) for a product of four varieties of bean ( L.) grown in a specific area of production, which is located in the Belluno district, Veneto region (N.E. of Italy). In the last decade, the 'Lamon bean' has been threatened by severe virus epidemics that have compromised its profitability. In this work, the full virome of seven bean samples showing different foliar symptoms was obtained by MinION sequencing. Evidence that emerged from sequencing was validated through RT-PCR and ELISA in a large number of plants, including different ecotypes of Lamon bean and wild herbaceous hosts that may represent a virus reservoir in the field. Results revealed the presence of bean common mosaic virus (BCMV), cucumber mosaic virus (CMV), peanut stunt virus (PSV), and bean yellow mosaic virus (BYMV), which often occurred as mixed infections. Moreover, both CMV and PSV were reported in association with strain-specific satellite RNAs (satRNAs). In conclusion, this work sheds light on the cause of the severe diseases affecting the 'Lamon bean' by exploitation of MinION sequencing.