Circulating miRNAs in the Plasma of Post-COVID-19 Patients with Typical Recovery and Those with Long-COVID Symptoms: Regulation of Immune Response-Associated Pathways

Following the acute phase of SARS-CoV-2 infection, certain individuals experience persistent symptoms referred to as long COVID. This study analyzed the patients categorized into three distinct groups: (1) individuals presenting rheumatological symptoms associated with long COVID, (2) patients who h...

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Bibliographic Details
Published in:Non-coding RNA 2024-09, Vol.10 (5), p.48
Main Authors: Timofeeva, Anna M, Nikitin, Artem O, Nevinsky, Georgy A
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Autoimmune diseases
B cells
catalytic antibodies
Cell cycle
COVID-19
Cytokines
Disease
Genes
Genomes
Guillain-Barre syndrome
Health aspects
Hydrolysis
Immune response
Infections
Influenza
long COVID
Medical research
Medicine, Experimental
MicroRNA
MicroRNAs
miRNA
Oligonucleotides
Patients
Plasma
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Viral antibodies
Viral infections
Viruses
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Summary:Following the acute phase of SARS-CoV-2 infection, certain individuals experience persistent symptoms referred to as long COVID. This study analyzed the patients categorized into three distinct groups: (1) individuals presenting rheumatological symptoms associated with long COVID, (2) patients who have successfully recovered from COVID-19, and (3) donors who have never contracted COVID-19. A notable decline in the expression of miR-200c-3p, miR-766-3p, and miR-142-3p was identified among patients exhibiting rheumatological symptoms of long COVID. The highest concentration of miR-142-3p was found in healthy donors. One potential way to reduce miRNA concentrations is through antibody-mediated hydrolysis. Not only can antibodies possessing RNA-hydrolyzing activity recognize the miRNA substrate specifically, but they also catalyze its hydrolysis. The analysis of the catalytic activity of plasma antibodies revealed that antibodies from patients with long COVID demonstrated lower hydrolysis activity against five fluorescently labeled oligonucleotide sequences corresponding to the Flu-miR-146b-5p, Flu-miR-766-3p, Flu-miR-4742-3p, and Flu-miR-142-3p miRNAs and increased activity against the Flu-miR-378a-3p miRNA compared to other patient groups. The changes in miRNA concentrations and antibody-mediated hydrolysis of miRNAs are assumed to have a complex regulatory mechanism that is linked to gene pathways associated with the immune system. We demonstrate that all six miRNAs under analysis are associated with a large number of signaling pathways associated with immune response-associated pathways.
ISSN:2311-553X
2311-553X
DOI:10.3390/ncrna10050048