Site‐specific fabrication of gold nanocluster‐based fluorescence photoswitch enabled by the dual roles of albumin proteins

Fluorescence photoswitch is becoming increasingly desirable for many applications, but its controllable fabrication still remains challenging yet. In this paper, a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers...

Full description

Saved in:
Bibliographic Details
Published in:Aggregate (Hoboken) 2023-02, Vol.4 (1), p.n/a
Main Authors: Zhong, Wencheng, Yan, Xiaojian, Qu, Shaohua, Shang, Li
Format: Article
Language:English
Subjects:
albumin protein
Binding sites
Chemistry
Dyes
fluorescence switch
gold nanoclusters
Information storage
inner filter effect
Light
Localization
Nonsteroidal anti-inflammatory drugs
photochrome
Proteins
Quantum dots
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fluorescence photoswitch is becoming increasingly desirable for many applications, but its controllable fabrication still remains challenging yet. In this paper, a new strategy is proposed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of fluorophore. As an example, we demonstrated the successful fabrication of a fluorescence photoswitch by incorporating both the photochromic diarylethene dye (CMTE) and red‐emitting fluorescent gold nanoclusters (AuNCs) into the specific domains of bovine serum albumin (BSA) in a highly controllable manner. Detailed spectral and photophysical characterisation showed that CMTE well‐retains the photochromic properties within the CMTE–BSA–AuNC construct, although its photoconversion rate is slightly retarded. Different from previously reported photoswitches, the fluorescence of the present system is mainly modulated via the inner filter effect (IFE) mechanism, which exhibits high switching efficiency with an on–off ratio up to 90%, reversible fluorescence response and good antifatigue performance. This work provides a new, generable albumin protein‐assisted strategy of fabricating advanced fluorescence photoswitch, which can find wide applications in biological, optical and information fields. A new strategy is developed to fabricate fluorescence photoswitch by harnessing dual roles of albumin proteins as both photochrome carriers and biotemplates of inorganic fluorophore, which exhibit high switching efficiency of 90%, reversible fluorescence response and good antifatigue performance.
ISSN:2692-4560
2766-8541
2692-4560
DOI:10.1002/agt2.245