Generation of a healthy heavy smoker patient-derived induced pluripotent stem cell line UHOMi007-A from peripheral blood mononuclear cells

Human pluripotent stem cells (hiPSC) represent a unique opportunity to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized healthy heavy smoker male donor free from emphysema or tobacco related disease. Peripheral blood mononuclear cells (PBMC...

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Bibliographic Details
Published in:Stem cell research 2024-06, Vol.77, p.103437, Article 103437
Main Authors: Ahmed, Engi, Fieldès, Mathieu, Bourguignon, Chloé, Mianné, Joffrey, Petit, Aurélie, Amel, Nasri, Foisset, Florent, Bourdais, Carine, Vachier, Isabelle, Assou, Said, De Vos, John, Bourdin, Arnaud
Format: Article
Language:English
Subjects:
Cell Differentiation
Cell Line
Cellular Reprogramming
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Leukocytes, Mononuclear - cytology
Leukocytes, Mononuclear - metabolism
Life Sciences
Male
Smokers
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Summary:Human pluripotent stem cells (hiPSC) represent a unique opportunity to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized healthy heavy smoker male donor free from emphysema or tobacco related disease. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi007-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing.
ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2024.103437