Loading…
Mycelium-bound lipase as skillful biocatalysts: Production of fatty acid concentrates from waste oils for the food industry
This study aimed to produce Rhizopus oryzae whole-cells having high lipolytic activity. The hydrolytic activity of 558.99 U.g−1 and total lipase activity of 7440.17 U was obtained in 72 h of cultivation using 30 g.L−1 of canola oil. Optimal activity was observed at pH 7.5 and 30 °C. Kinetic paramete...
Saved in:
Published in: | Catalysis communications 2023-11, Vol.184, p.106787, Article 106787 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | This study aimed to produce Rhizopus oryzae whole-cells having high lipolytic activity. The hydrolytic activity of 558.99 U.g−1 and total lipase activity of 7440.17 U was obtained in 72 h of cultivation using 30 g.L−1 of canola oil. Optimal activity was observed at pH 7.5 and 30 °C. Kinetic parameters were determined through olive oil hydrolysis (Km = 59.96 mM, Vmax = 344.09 μmol.g−1.min−1). Thermal inactivation constant and half-life were 0.15 h−1 and 4.62 h at 50 °C. Hydrolysis of vegetable oils achieved 80% yield in 24 h. These results suggest that the biocatalyst shows the potential to produce free fatty acids of great industrial interest.
[Display omitted]
•Rhizopus oryzae cells showed elevated lipase hydrolytic activity (max. 558.99 U.g−1).•Mycelium-bound lipase showed good thermal stability at 50 °C (t1/2 = 4.62 h).•Whole cells bioprocess presented an industrial potential for vegetable oils hydrolysis. |
---|---|
ISSN: | 1566-7367 1873-3905 |
DOI: | 10.1016/j.catcom.2023.106787 |