Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction

The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism. Blood samples were collected from the B...

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Bibliographic Details
Published in:Veterinary World 2020-05, Vol.13 (5), p.879-883
Main Authors: Utomo, Budi, Putranto, Emmanuel Djoko, Fadholly, Amaq
Format: Article
Language:English
Subjects:
Alleles
Beef cattle
Cattle
Cross-breeding
EDTA
Enzymes
Follicle-stimulating hormone
follicle-stimulating hormone receptors
Gene frequency
Gene polymorphism
Genes
Genetic aspects
Genetic polymorphisms
hypofunction
Livestock
Luteinizing hormone
madrasin
Polymerase chain reaction
Polymorphism
Restriction fragment length polymorphism
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Summary:The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism. Blood samples were collected from the Bangkalan regency in Indonesia. DNA was isolated and purified following the extraction protocol of polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. Our results showed that the FSH gene had a band length of 310 bp and produce two alleles (A and B) with restriction enzymes at 250 bp, 230 bp, and 145 bp. Furthermore, the FSHR gene had a band length of 303 bp and produced two homozygous genotypes: GG at bp 239 and CC at bp 188. Based on these differences, there was no change in allele frequency and genotype between Madura and Madrasin cattle due to crossbreeding with Limousin cattle. Thus, further detailed investigations of Madrasin cattle are required to elucidate the profile of the LH and LHR genes.
ISSN:0972-8988
2231-0916
DOI:10.14202/vetworld.2020.879-883