Omics analyses and biochemical study of Phlebiopsis gigantea elucidate its degradation strategy of wood extractives

Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degra...

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Bibliographic Details
Published in:Scientific reports 2021-06, Vol.11 (1), p.12528-12528, Article 12528
Main Authors: Iwata, Mana, Gutiérrez, Ana, Marques, Gisela, Sabat, Grzegorz, Kersten, Philip J., Cullen, Daniel, Bhatnagar, Jennifer M., Yadav, Jagjit, Lipzen, Anna, Yoshinaga, Yuko, Sharma, Aditi, Adam, Catherine, Daum, Christopher, Ng, Vivian, Grigoriev, Igor V., Hori, Chiaki
Format: Article
Language:English
Subjects:
631/326
631/326/193
BASIC BIOLOGICAL SCIENCES
Biodegradation
Cellulose
Colonization
Conifers
Decay fungi
Enzymes
Gene expression
Hemicellulose
Humanities and Social Sciences
Lipophilic
multidisciplinary
Phlebiopsis gigantea
Proteomes
Science
Science (multidisciplinary)
Softwoods
Steroid hormones
Transcriptomes
Wood
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Summary:Wood extractives, solvent-soluble fractions of woody biomass, are considered to be a factor impeding or excluding fungal colonization on the freshly harvested conifers. Among wood decay fungi, the basidiomycete Phlebiopsis gigantea has evolved a unique enzyme system to efficiently transform or degrade conifer extractives but little is known about the mechanism(s). In this study, to clarify the mechanism(s) of softwood degradation, we examined the transcriptome, proteome, and metabolome of P. gigantea when grown on defined media containing microcrystalline cellulose and pine sapwood extractives. Beyond the conventional enzymes often associated with cellulose, hemicellulose and lignin degradation, an array of enzymes implicated in the metabolism of softwood lipophilic extractives such as fatty and resin acids, steroids and glycerides was significantly up-regulated. Among these, a highly expressed and inducible lipase is likely responsible for lipophilic extractive degradation, based on its extracellular location and our characterization of the recombinant enzyme. Our results provide insight into physiological roles of extractives in the interaction between wood and fungi.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-91756-5