Bioefficacy of Nga-Mon ( Perilla frutescens ) Fresh and Dry Leaf: Assessment of Antioxidant, Antimutagenicity, and Anti-Inflammatory Potential

• Published in: Plants (Basel) 2023-06, Vol.12 (11), p.2210
• Main Authors: Tantipaiboonwong, Payungsak, Pintha, Komsak, Chaiwangyen, Wittaya, Suttajit, Maitree, Khanaree, Chakkrit, Khantamat, Orawan
• Format: Article
• Language: English
• Subjects: Analysis; anti-inflammation; Anti-inflammatory agents; Antimutagenicity; antioxidant; Antioxidants; Biological activity; Biological effects; Carcinogens; Cellular manufacture; Chemical properties; Cytokines; Cytotoxicity; Ferulic acid; Free radicals; Health aspects; Inflammation; Interleukin 6; Lamiaceae; Leaves; Lipopolysaccharides; Materia medica, Vegetable; Metabolism; NF-κB protein; Nga-mon; Nitric oxide; Nitric-oxide synthase; Oxidative stress; Perilla frutescens; Phenols; Phosphorylation; Phytochemicals; Plant extracts; Polyphenols; Proteins; Rosmarinic acid; Scavenging; Translocation; Tumor necrosis factor-TNF; Tumor necrosis factor-α
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12112210

Perilla leaves are known to be a rich source of polyphenols, which have been shown to exhibit various biological effects. This study aimed to compare the bioefficacies and bioactivities of fresh (PLE ) and dry (PLE ) Thai perilla (Nga-mon) leaf extracts. Phytochemical analysis indicated that both PLE and PLE were abundant in rosmarinic acid and bioactive phenolic compounds. PLE , which had higher levels of rosmarinic acid but lower levels of ferulic acid and luteolin than PLE , exhibited greater effectiveness in a free radical scavenging assay. Furthermore, both extracts were found to suppress intracellular ROS generation and exhibit antimutagenic activity against food-borne carcinogens in . They also attenuated lipopolysaccharide-induced inflammation in RAW 264.7 cells by inhibiting the expression of nitric oxide, iNOS, COX-2, TNF-α, IL-1β, and IL-6 through the suppression of NF-κB activation and translocation. However, PLE exhibited a higher ability to suppress cellular ROS production and higher antimutagenic and anti-inflammatory activities than PLE , which can be attributed to its combination of phytochemical components. Overall, PLE and PLE have the potential to serve as natural bioactive antioxidant, antimutagenic, and anti-inflammatory agents to achieve potential health benefits.