Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production

(formerly known as ) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant...

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Bibliographic Details
Published in:Metabolites 2022-04, Vol.12 (4), p.346
Main Authors: Bustos, Cristina, Quezada, Johan, Veas, Rhonda, Altamirano, Claudia, Braun-Galleani, Stephanie, Fickers, Patrick, Berrios, Julio
Format: Article
Language:English
Subjects:
Alcohol
Biochemistry
Biomass
Bioreactors
Biotechnologie
Biotechnology
Carbon
cell engineering
Dehydrogenases
Endocrinology, Diabetes and Metabolism
Gene expression
Genetic engineering
Glycerol
Komagataella phaffii
Life sciences
Metabolic engineering
Metabolism
Metabolites
Molecular Biology
Oxidative stress
Pichia pastoris
Productivity
Protein folding
Proteins
recombinant protein
Review
Sciences du vivant
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Summary:(formerly known as ) has become an increasingly important microorganism for recombinant protein production. This yeast species has gained high interest in an industrial setting for the production of a wide range of proteins, including enzymes and biopharmaceuticals. During the last decades, relevant bioprocess progress has been achieved in order to increase recombinant protein productivity and to reduce production costs. More recently, the improvement of cell features and performance has also been considered for this aim, and promising strategies with a direct and substantial impact on protein productivity have been reported. In this review, cell engineering approaches including metabolic engineering and energy supply, transcription factor modulation, and manipulation of routes involved in folding and secretion of recombinant protein are discussed. A lack of studies performed at the higher-scale bioreactor involving optimisation of cultivation parameters is also evidenced, which highlights new research aims to be considered.
ISSN:2218-1989
2218-1989
DOI:10.3390/metabo12040346