Investigating the effect of Er,Cr:YSGG laser agitation of sodium hypochlorite on the removal of mature biofilm in the complex root canal systems using atomic force microscopy

Background. Endodontic infections caused by remaining biofilm following disinfection with chemical fluids encourage secondary bacterial infection; hence, employing laser pulses to activate the fluids is advised to improve microbial biofilm clearance. This study investigated the performance of Er,Cr:...

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Bibliographic Details
Published in:Journal of dental research, dental clinics, dental prospects dental clinics, dental prospects, 2023, Vol.17 (3), p.154-161
Main Authors: Ibrahim, Ghufran Ismail, Jawad, Hussein Ali
Format: Article
Language:English
Subjects:
5.25% sodium hypochlorite
Agitation
atomic force microscope
Atomic force microscopy
Bacteria
Bacterial infections
biofilm
Biofilms
Cavitation
cr:ysgg laser
Dental roots
Disinfectants
Disinfection
Disinfection & disinfectants
Endodontics
enterococcus faecalis
Gallium
Investigations
Lasers
Lavage
Microscopy
Molars
passive ultrasonic activation
Proteolysis
Root canals
Sodium
Sodium hypochlorite
Streaming
Teeth
Yttrium
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Summary:Background. Endodontic infections caused by remaining biofilm following disinfection with chemical fluids encourage secondary bacterial infection; hence, employing laser pulses to activate the fluids is advised to improve microbial biofilm clearance. This study investigated the performance of Er,Cr:YSGG laser in photon-induced photoacoustic streaming (PIPS) agitation of 5.25% sodium hypochlorite (NaOCl) to enhance the removal of mature Enterococcus faecalis (E. faecalis) biofilms in complex root canal systems. Methods. The mesial roots of the lower first and second molars were separated and inoculated with E. faecalis bacteria for 30 days. The roots were irrigated with 5.25% NaOCl, some of them were agitated with passive ultrasonic irrigation (PUI), and the other roots were agitated by Er,Cr:YSGG laser using PIPS at 60 µs/pulse, 5 Hz, and 0.25, 0.5, 0.75, 1, and 1.25 W. An atomic force microscope (AFM) was used as a new method to obtain the results in the isthmus area; the results that have been obtained from each group were compared with each other. ANOVA was utilized to compare the means of the test groups. Results. Based on the AFM and SEM analyses, laser agitation and passive ultrasonic activation groups have shown higher antimicrobial efficacy than the conventional syringe irrigation group (P
ISSN:2008-210X
2008-2118
DOI:10.34172/joddd.2023.40463