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Towards the physical map of the Trypanosoma cruzi nuclear genome: construction of YAC and BAC libraries of the reference clone T. cruzi CL-Brener
Strategies to construct the physical map of the Trypanosoma cruzi nuclear genome have to capitalize on three main advantages of the parasite genome, namely (a) its small size, (b) the fact that all chromosomes can be defined, and many of them can be isolated by pulse field gel electrophoresis, and (...
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Published in: | Memórias do Instituto Oswaldo Cruz 1997-11, Vol.92 (6), p.843-852 |
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Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | Strategies to construct the physical map of the Trypanosoma cruzi
nuclear genome have to capitalize on three main advantages of the
parasite genome, namely (a) its small size, (b) the fact that all
chromosomes can be defined, and many of them can be isolated by pulse
field gel electrophoresis, and (c) the fact that simple Southern blots
of electrophoretic karyotypes can be used to map sequence tagged sites
and expressed sequence tags to chromosomal bands. A major drawback to
cope with is the complexity of T. cruzi genetics, that hinders the
construction of a comprehensive genetic map. As a first step towards
physical mapping, we report the construction and partial
characterization of a T. cruzi CL-Brener genomic library in yeast
artificial chromosomes (YACs) that consists of 2,770 individual YACs
with a mean insert size of 365 kb encompassing around 10 genomic
equivalents. Two libraries in bacterial artificial chromosomes (BACs)
have been constructed, BACI and BACII. Both libraries represent about
three genome equivalents. A third BAC library (BAC III) is being
constructed. YACs and BACs are invaluable tools for physical mapping.
More generally, they have to be considered as a common resource for
research in Chagas disease. |
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ISSN: | 1678-8060 0074-0276 0074-0276 1678-8060 |
DOI: | 10.1590/S0074-02761997000600021 |