Consolidated Bioprocess for Bioethanol Production from Raw Flour of Brosimum alicastrum Seeds Using the Native Strain of Trametes hirsuta Bm-2

Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of (Bm-2) was evaluated for bioethanol...

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Published in:Microorganisms (Basel) 2019-10, Vol.7 (11), p.483
Main Authors: Olguin-Maciel, Edgar, Larqué-Saavedra, Alfonso, Lappe-Oliveras, Patricia E, Barahona-Pérez, Luis F, Alzate-Gaviria, Liliana, Chablé-Villacis, Rubí, Domínguez-Maldonado, Jorge, Pacheco-Catalán, Daniella, Ruíz, Hector A, Tapia-Tussell, Raúl
Format: Article
Language:English
Subjects:
Biofuels
Biomass
Bioprocessing
Biorefineries
Brosimum alicastrum
Climate change
consolidated bioprocess
Consolidation
Enzymes
Ethanol
Fermentation
Flour
Fungi
Glucose
Iodine
Microorganisms
Saccharification
Seeds
Starch
Trametes hirsuta
white rot fungi
Yeast
α-amylase
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Summary:Consolidated bioprocessing (CBP), which integrates biological pretreatment, enzyme production, saccharification, and fermentation, is a promising operational strategy for cost-effective ethanol production from biomass. In this study, the use of a native strain of (Bm-2) was evaluated for bioethanol production from in a CBP. The raw seed flour obtained from the ramon tree contained 61% of starch, indicating its potential as a raw material for bioethanol production. Quantitative assays revealed that the Bm-2 strain produced the amylase enzyme with activity of 193.85 U/mL. The Bm-2 strain showed high tolerance to ethanol stress and was capable of directly producing ethanol from raw flour at a concentration of 13 g/L, with a production yield of 123.4 mL/kg flour. This study demonstrates the potential of Bm-2 for starch-based ethanol production in a consolidated bioprocess to be implemented in the biofuel industry. The residual biomass after fermentation showed an average protein content of 22.5%, suggesting that it could also be considered as a valuable biorefinery co-product for animal feeding.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms7110483