Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe

Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutation...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2017-04, Vol.17 (5), p.961
Main Authors: Zhang, Cheng, Chen, Yiping, Liang, Xinmiao, Zhang, Guanhua, Ma, Hong, Nie, Leng, Wang, Yu
Format: Article
Language:English
Subjects:
Biochemistry
Deoxyribonucleic acid
Diagnosis
DNA
DNA polymerase
Drug resistance
Efficiency
Fluorescence
HBV DNA
Hepatitis
Hepatitis B
Imaging
Interferon
Microscopy
Mutation
Patients
quantum dot
Quantum dots
sequencing
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Summary:Quantum dots (QDs) are semiconductor nanoparticles with a diameter of less than 10 nm, which have been widely used as fluorescent probes in biochemical analysis and vivo imaging because of their excellent optical properties. Sensitive and convenient detection of hepatitis B virus (HBV) gene mutations is important in clinical diagnosis. Therefore, we developed a sensitive, low-cost and convenient QDs-mediated fluorescent method for the detection of HBV gene mutations in real serum samples from chronic hepatitis B (CHB) patients who had received lamivudine or telbivudine antiviral therapy. We also evaluated the efficiency of this method for the detection of drug-resistant mutations compared with direct sequencing. In CHB, HBV DNA from the serum samples of patients with poor response or virological breakthrough can be hybridized to probes containing the M204I mutation to visualize fluorescence under fluorescence microscopy, where fluorescence intensity is related to the virus load, in our method. At present, the limits of the method used to detect HBV genetic variations by fluorescence quantum dots is 10³ IU/mL. These results show that QDs can be used as fluorescent probes to detect viral HBV DNA polymerase gene variation, and is a simple readout system without complex and expensive instruments, which provides an attractive platform for the detection of HBV M204I mutation.
ISSN:1424-8220
1424-8220
DOI:10.3390/s17050961