Growth and Genome Features of Non-O1/O139 Vibrio cholerae Isolated from Three Species of Common Freshwater Fish

Vibrio cholerae is the etiological agent of cholera in humans. The bacterium is frequently detected in aquatic products worldwide. However, the current literature on the genome evolution of V. cholerae of aquatic animal origins is limited. Here, we firstly characterized the growth and genome feature...

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Bibliographic Details
Published in:Diversity (Basel) 2024-05, Vol.16 (5), p.268
Main Authors: Qin, Xinchi, Yang, Lianzhi, Xu, Yingwei, Xie, Lu, Wang, Yongjie, Chen, Lanming
Format: Article
Language:English
Subjects:
Antibiotic resistance
Antibiotics
Aquatic animals
Bacteria
Cholera
CRISPR
Drinking water
Drug resistance
Epidemics
Fish
Freshwater fish
Gene sequencing
Genes
genome evolution
Genomes
Genomic analysis
Genomic islands
Insertion sequences
mobile genetic elements
Phenotypes
Phylogenetics
Prophages
Quality control
Sodium chloride
Toxins
Vibrio cholerae
Virulence
Waterborne diseases
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Summary:Vibrio cholerae is the etiological agent of cholera in humans. The bacterium is frequently detected in aquatic products worldwide. However, the current literature on the genome evolution of V. cholerae of aquatic animal origins is limited. Here, we firstly characterized the growth and genome features of V. cholerae isolates with different resistance phenotypes from three species of common freshwater fish. The results revealed that the non-O1/O139 V. cholerae isolates (n = 4) were halophilic and grew optimally at 2% NaCl and pH 8.0. Their draft genome sequences were 3.89 Mb–4.15 Mb with an average GC content of 47.35–47.63%. Approximately 3366–3561 genes were predicted to encode proteins, but 14.9–17.3% of them were of an unknown function. A number of strain-specific genes (n = 221–311) were found in the four V. cholerae isolates, 3 of which belonged to none of any of the known sequence types (STs). Several putative mobile genetic elements (MGEs) existed in the V. cholerae isolates, including genomic islands (n = 4–9), prophages (n = 0–3), integrons (n = 1–1), and insertion sequences (n = 0–3). Notably, CRISPR-Cas system arrays (n = 2–10) were found in the V. cholerae genomes, whereby the potential immunity defense system could be active. Comparative genomic analyses also revealed many putative virulence-associated genes (n = 106–122) and antibiotic resistance-related genes (n = 6–9). Overall, the results of this study demonstrate the bacterial broader-spectrum growth traits and fill prior gaps in the genomes of V. cholerae originating from freshwater fish.
ISSN:1424-2818
1424-2818
DOI:10.3390/d16050268