Association of kinesin family member 2A with increased disease risk, deteriorative clinical characteristics, and shorter survival profiles in acute myeloid leukemia

• Published in: Brazilian journal of medical and biological research 2021-01, Vol.54 (2), p.e9173-e9173
• Main Authors: Ding, Tianling, Li, Jialing, Sun, Jianhong, Fan, Xiaoman, Shi, Chunli, Zhou, Dong, Deng, Ruoyu
• Format: Article
• Language: English
• Subjects: Acute myeloid leukemia; Adult; Apoptosis; BIOLOGY; Biomarkers, Tumor - genetics; Bone marrow; Bone marrow transplantation; CD34 antigen; Cell apoptosis; Cell growth; Cell Proliferation; Clinical characteristics; Female; Gene Knockdown Techniques; Hematopoietic stem cells; HL-60 Cells; Humans; Karyotypes; Kinesin; Kinesin - genetics; Kinesin family member 2A; Leukemia; Leukemia, Myeloid, Acute - diagnosis; Leukemia, Myeloid, Acute - genetics; Leukocytes; Male; Medical prognosis; MEDICINE, RESEARCH & EXPERIMENTAL; Middle Aged; Myeloid leukemia; Patients; Polymerase chain reaction; Remission; Risk Factors; Stem cell transplantation; Survival; Survival Rate
• ISSN: 0100-879X; 1414-431X; 1414-431X; 1678-4510
• DOI: 10.1590/1414-431x20209173

This study aimed to explore the correlation of kinesin family member 2A (KIF2A) expression with disease risk, clinical characteristics, and prognosis of acute myeloid leukemia (AML), and investigate the effect of KIF2A knockdown on AML cell activities in vitro. Bone marrow samples were collected from 176 AML patients and 40 healthy donors, and KIF2A expression was measured by real-time quantitative polymerase chain reaction. Treatment response, event-free survival (EFS), and overall survival (OS) were assessed in AML patients. In vitro, KIF2A expression in AML cell lines and CD34+ cells (from healthy donors) was measured, and the effect of KIF2A knockdown on AML cell proliferation and apoptosis in HL-60 and KG-1 cells was detected. KIF2A expression was greater in AML patients compared to healthy donors, and receiver operating characteristic curve indicated that KIF2A expression predicted increased AML risk (area under curve: 0.793 (95%CI: 0.724-0.826)). In AML patients, KIF2A expression positively correlated with white blood cells, monosomal karyotype, and high risk stratification. Furthermore, no correlation of KIF2A expression with complete remission or hematopoietic stem cell transplantation was found. Kaplan-Meier curves showed that KIF2A expression was negatively correlated with EFS and OS. In vitro experiments showed that KIF2A was overexpressed in AML cell lines (KG-1, HL-60, ME-1, and HT-93) compared to CD34+ cells, moreover, cell proliferation was reduced but apoptosis was increased by KIF2A knockdown in HL-60 and KG-1 cells. In conclusion, KIF2A showed potential to be a biomarker and treatment target in AML.