Impacts of Different Prenatal Supplementation Strategies on the Plasma Metabolome of Bulls in the Rearing and Finishing Phase

• Published in: Metabolites 2023-02, Vol.13 (2), p.259
• Main Authors: Polizel, Guilherme Henrique Gebim, Fernandes, Arícia Christofaro, Furlan, Édison, Prati, Barbara Carolina Teixeira, Ferraz, José Bento Sterman, Santana, Miguel Henrique de Almeida
• Format: Article
• Language: English
• Subjects: Amino acids; Beef cattle; Blood plasma; Bulls; Chemical properties; Chromatography; Datasets; Dietary supplements; fetal programming; Food and nutrition; Histidine; Machine learning; Males; Mass spectrometry; Metabolic pathways; Metabolism; Metabolites; metabolomics; nutrigenomics; Nutrition; Physiological aspects; Plasma; Pregnancy; Prenatal experience; Principal components analysis; Proteins; Scientific imaging; Software; Veterinary obstetrics; Weaning
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo13020259

This study investigated the effects of maternal nutrition on the plasma metabolome of Nellore bulls in the rearing and finishing phases, and metabolic differences between these phases. For this study, three nutritional approaches were used in 126 cows during pregnancy: NP-(control) mineral supplementation; PP-protein-energy supplementation in the final third; and FP-protein-energy supplementation during the entire pregnancy. We collected blood samples from male offspring in the rearing (450 ± 28 days old) and finishing phases (660 ± 28 days old). The blood was processed, and from plasma samples, we performed the targeted metabolome analysis (AbsoluteIDQ p180 Kit). Multiple linear regression, principal component analysis (PCA), repeated measures analysis over time, and an enrichment analysis were performed. PCA showed an overlap of treatments and time clusters in the analyses. We identified significant metabolites among the treatments (rearing phase = six metabolites; finishing phase = three metabolites) and over time (21 metabolites). No significant metabolic pathways were found in the finishing phase, however, we found significant pathways in the rearing phase (Arginine biosynthesis and Histidine metabolism). Thus, prenatal nutrition impacted on plasma metabolome of bulls during the rearing and finishing phase and the different production stages showed an effect on the metabolic levels of bulls.