MicroRNA‐374b mediates the initiation of non‐small cell lung cancer by regulating ITGB1 and p53 expressions

Background Previous studies have shown that microRNAs (miRNAs) play important roles in the pathogenesis of human cancers. This study aims to clarify the role of miR‐374b in non‐small cell lung cancer (NSCLC). Methods In this study, RT‐qPCR and western blot analysis were used to measure mRNA and prot...

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Bibliographic Details
Published in:Thoracic cancer 2020-06, Vol.11 (6), p.1670-1678
Main Authors: Zhao, Meng, Tong, Chuntang, Hao, Zerui, Zhao, Ruixing, Wang, Liming
Format: Article
Language:English
Subjects:
Apoptosis
Binding sites
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Carcinoma, Non-Small-Cell Lung - genetics
Carcinoma, Non-Small-Cell Lung - metabolism
Carcinoma, Non-Small-Cell Lung - pathology
Cell adhesion & migration
Cell growth
Cell Movement
Cell Proliferation
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Genes
Humans
Integrin beta1 - genetics
Integrin beta1 - metabolism
ITGB1
Lung cancer
Lung Neoplasms - genetics
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Lymphatic system
Male
Medical research
Metastasis
MicroRNAs
MicroRNAs - genetics
Middle Aged
miR‐374b
non‐small cell lung cancer
Original
p53
Pancreatic cancer
Prognosis
Proteins
Tumor Cells, Cultured
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
Tumorigenesis
Tumors
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Summary:Background Previous studies have shown that microRNAs (miRNAs) play important roles in the pathogenesis of human cancers. This study aims to clarify the role of miR‐374b in non‐small cell lung cancer (NSCLC). Methods In this study, RT‐qPCR and western blot analysis were used to measure mRNA and protein expression. The regulatory mechanism of miR‐374b/ITGB1 was investigated by dual‐luciferase reporter, CCK‐8, and transwell assays. Results MiR‐374b expression was reduced in NSCLC tissues and associated with lymph node metastasis, tumor stage and prognosis in NSCLC patients. Functionally, overexpression of miR‐374b inhibited cell viability and metastasis in NSCLC. In addition, miR‐374b blocked EMT and promoted p53 expression in NSCLC. MiR‐374b was found to directly target ITGB1. Furthermore, upregulation of ITGB1 weakened the antitumor effect of miR‐374b in NSCLC. Conclusions MiR‐374b inhibits the tumorigenesis of NSCLC by downregulating ITGB1 and upregulating p53.
ISSN:1759-7706
1759-7714
DOI:10.1111/1759-7714.13457