CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection

• Published in: Memórias do Instituto Oswaldo Cruz 1995-01, Vol.90 (1), p.51-58
• Main Authors: Lezama-Dávila, C M, Gallagher, G
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Animals; CD4-CD8 Ratio; CD4-Positive T-Lymphocytes - immunology; CD4-Positive T-Lymphocytes - parasitology; CD8-Positive T-Lymphocytes - immunology; CD8-Positive T-Lymphocytes - parasitology; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Female; Immunoglobulin G - analysis; Leishmania m. mexicana; Leishmania mexicana - immunology; Leishmaniasis, Cutaneous - immunology; liposomes monoclonal antibodies; Male; Mice; Mice, Inbred CBA; PARASITOLOGY; T cell-subpopulations; TROPICAL MEDICINE; Vaccination
• ISSN: 0074-0276; 1678-8060; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02761995000100012

We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania.