CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection

We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were us...

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Published in:Memórias do Instituto Oswaldo Cruz 1995-01, Vol.90 (1), p.51-58
Main Authors: Lezama-Dávila, C M, Gallagher, G
Format: Article
Language:English
Subjects:
AIDS/HIV
Animals
CD4-CD8 Ratio
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - parasitology
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - parasitology
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G - analysis
Leishmania m. mexicana
Leishmania mexicana - immunology
Leishmaniasis, Cutaneous - immunology
liposomes monoclonal antibodies
Male
Mice
Mice, Inbred CBA
PARASITOLOGY
T cell-subpopulations
TROPICAL MEDICINE
Vaccination
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Gallagher, G
description We studied the role of CD4+, CD8+, CD4- CD8- T cells and IgG anti-Leishmania after infection or vaccination in the CBA/ca mouse. Mice were either infected with L. m. mexicana promastigotes or vaccinated with parasite-membrane antigens incorporated into liposomes. Successfully vaccinated mice were used as cell-donors in adoptive transfer experiments. Naive, syngeneic recipients received highly-enriched CD4+, CD8+ or CD4- CD8- T cells from those two set of donors and challenged with live parasites. Our results showed that, both CD4+ and CD8+ T cells from infected or vaccinated donors conferred significant disease-resistance to naive recipients. In addition, adoptive transfer of CD4- CD8- T cells from vaccinated donors significantly delayed lesion growth in recipient mice. We concluded that vaccination of CBA mice correlates with the induction of protective CD4+, CD8+ and CD4- CD8- T cells and the synthesis of IgG anti-Leishmania.
doi_str_mv 10.1590/S0074-02761995000100012
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subjects AIDS/HIV
Animals
CD4-CD8 Ratio
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - parasitology
CD8-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - parasitology
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Immunoglobulin G - analysis
Leishmania m. mexicana
Leishmania mexicana - immunology
Leishmaniasis, Cutaneous - immunology
liposomes monoclonal antibodies
Male
Mice
Mice, Inbred CBA
PARASITOLOGY
T cell-subpopulations
TROPICAL MEDICINE
Vaccination
title CD4+, CD8+ and CD4- CD8- T cell-subsets can confer protection against Leishmania m. mexicana infection
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