Berry-Enriched Diet in Salt-Sensitive Hypertensive Rats: Metabolic Fate of (Poly)Phenols and the Role of Gut Microbiota

• Published in: Nutrients 2019-11, Vol.11 (11), p.2634
• Main Authors: Gomes, Andreia, Oudot, Carole, Macià, Alba, Foito, Alexandre, Carregosa, Diogo, Stewart, Derek, Van de Wiele, Tom, Berry, David, Motilva, Maria-José, Brenner, Catherine, Dos Santos, Cláudia Nunes
• Format: Article
• Language: English
• Subjects: Animals; Berries; Blood pressure; cardiovascular; Diet; Disease; dysbiosis; Dysbiosis - metabolism; Fatty acids; Feces - microbiology; Fruit; Fruits; Gastrointestinal Microbiome - genetics; Gastrointestinal Microbiome - physiology; Glycosides - metabolism; gut metabolism; high salt intake; Intestinal microflora; Life Sciences; Male; Metabolism; Metabolites; Microbiota; Phenols; Phenols - analysis; Phenols - metabolism; Phytochemicals - analysis; Phytochemicals - metabolism; Polyphenols; Rats; Rats, Inbred Dahl; Sodium, Dietary
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11112634

Diets rich in (poly)phenols are associated with a reduced reduction in the incidence of cardiovascular disorders. While the absorption and metabolism of (poly)phenols has been described, it is not clear how their metabolic fate is affected under pathological conditions. This study evaluated the metabolic fate of berry (poly)phenols in an in vivo model of hypertension as well as the associated microbiota response. Dahl salt-sensitive rats were fed either a low-salt diet (0.26% NaCl) or a high-salt diet (8% NaCl), with or without a berry mixture (blueberries, blackberries, raspberries, Portuguese crowberry and strawberry tree fruit) for 9 weeks. The salt-enriched diet promoted an increase in the urinary excretion of berry (poly)phenol metabolites, while the abundance of these metabolites decreased in faeces, as revealed by UPLC-MS/MS. Moreover, salt and berries modulated gut microbiota composition as demonstrated by 16S rRNA analysis. Some changes in the microbiota composition were associated with the high-salt diet and revealed an expansion of the families and . However, this effect was mitigated by the dietary supplementation with berries. Alterations in the metabolic fate of (poly)phenols occur in parallel with the modulation of gut microbiota in hypertensive rats. Thus, beneficial effects of (poly)phenols could be related with these interlinked modifications, between metabolites and microbiota environments.